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新乡学院生命科学技术学院基因工程课件 Tools for DNA Cloning。
Cloning and Characterization of an Endoglucanase Gene from Actinomyces sp. Korean Native Goat 40
Korean Native Goat Actinomyces sp Endo-β-1 4-glucanase Cellulase
2016/5/20
A gene from Actinomyces sp. Korean native goat (KNG) 40 that encodes an endo-β-1,4-glucanase, EG1, was cloned and expressed in Escherichia coli (E. coli) DH5α. Recombinant plasmid DNA from a positive ...
Molecular Cloning, mRNA Expression,and Localization of the G-protein Subunit Galphaq in Sheep Testis and Epididymis
Epididymis G-protein Subunit Galphaq [GNAQ] Sheep Testis
2016/12/28
The reproductive function of G-protein subunit Galphaq (GNAQ), a member of the G protein alpha subunit family, has been extensively studied in humans and rats. However, no data is available on its sta...
Molecular Cloning and Characterization of a New C-type Lysozyme Gene from Yak Mammary Tissue
Yak Mammary Tissue Milk Lysozyme Recombinant Protein
2016/5/19
Milk lysozyme is the ubiquitous enzyme in milk of mammals. In this study, the cDNA sequence of a new chicken-type (c-type) milk lysozyme gene (YML), was cloned from yak mammary gland tissue. A 444 bp ...
Cloning and characterization of thioredoxin peroxidase (TPx) in {\it Onchocerca volvulus\/}
Infection silk worm parasites prevent infection animal models and gene
2014/12/11
About 400 million individuals worldwide are currently infected with filarial parasites. To find a cure or prevention for the infections, studies of the mechanisms of pathogenesis and the survival stra...
Cloning and characterization of a new gene involved in lymphocyte activation
The immune system mouse models cow in vitro lymphocyte activation immunology lymphocyte activated gene
2014/12/11
Cattle represent an economically important species whose immune system seems to depart from the standard human and murine models. Little has been documented about bovine lymphocyte activation in vitro...
Towards the isolation of esterase and amidase catalytic antibodies: Examination of germinal center diversity by cloning and sequence analysis of genes encoding monoclonal antibodies elicited by a transition state analogue
Esterase catalytic antibodies amidase amide ester hydrolysis reaction hybrid cells B cells the TSA induced antibodies genes cells cell lines
2014/12/11
In this study, a transition state analogue (TSA) approach was employed for the isolation of catalytic antibodies with esterase or amidase activity. The TSA used in this study, phosphonamidate, mimics ...
Cloning and characterization of GUKHolder, a novel synaptically expressed protein that interacts with Discs -Large and SCRIBBLE at the Drosophila neuromuscular junction
Neurons spatial arrangement protein tumor protein complex enzyme
2014/12/11
Synaptic transmission between a neuron and its target is crucially dependent upon the precise spatial arrangement of proteins in the pre- and postsynaptic apparatus. PDZ domain-containing proteins suc...
Cloning and functional characterization of the zebrafish mutation belladonna
Belladonna zebrafish mutations mutagenic screen and identify genes the retina the nerve cross the pupil
2014/12/11
The zebrafish belladonna (bel) mutation was identified in a large-scale mutagenesis screen to identify genes involved in retino-tectal pathfinding in Tubingen, Germany. In bel mutants, after exiting t...
Molecular cloning of the soybean phototropins
Phototropins Photoreceptors Arabidopsis Soybean Photosynthesis Agrobacterium Tumefaciens
2015/5/22
The phototropin photoreceptors are important regulators of plant growth and development and can therefore affect the photosynthetic activity of plants. Phototropin1 and Phototropin2 are versatile prot...
Cloning and Molecular Characterization of Porcine β-casein Gene (CNS2)
Lactogenic Hormones HC11 Cells TATA Box Milk Protein Porcine Casein Gene
2016/5/9
The production of therapeutic proteins from transgenic animals is one of the most important successes of animal biotechnology. Milk is presently the most mature system for production of therapeutic pr...
Interspecies Somatic Cell Nuclear Transfer Technique for Researching Dog Cloning and Embryonic Stem Cells
Canine Interspecies Porcine Somatic cell Nuclear Transfer
2016/5/5
Large quantities of high-quality recipient oocytes with uniform cytoplasm are needed for research in the promising field of somatic cell nuclear transfer (SCNT) and embryonic stem cell research. In ca...
Cloning and Distribution of Facilitative Glucose Transporter 2 (SLC2A2) in Pigs
GLUT2 Molecular Cloning Small Intestine Pig
2016/5/5
Glucose is the main energy source for mammalian cells and its absorption is co-mediated by two different families of glucose transporters, sodium/glucose co-transporters (SGLTs) and facilitative gluco...
Cloning and Expression of FSHb Gene and the Effect of FSHβ on the mRNA Levels of FSHR in the Local Chicken
Chicken FSHb Gene Expression FSH-receptor
2016/5/4
Follicle-stimulating hormone (FSH) is a pituitary glycoprotein hormone that is encoded by separate alpha- and beta-subunit genes. It plays a key role in stimulating and regulating ovarian follicular d...