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应用LH-PCR研究党参、麻黄和独一味内生细菌多样性
药用植物 细菌多样性 免培养 LH-PCR
2013/9/17
通过直接提取药用植物样品的总DNA,采用长度多态片段PCR (length heterogeneity PCR, LH-PCR)技术研究四川省甘孜藏族自治州的党参、麻黄和独一味3种药用植物内生细菌多样性.结果表明: 同种植物根、茎、叶的LH-PCR图谱相似度很高,条带丰富度差别不大;但不同植物样品之间的差异较大.党参的植物样品条带丰富度最大,麻黄次之,独一味最低.3种药用植物中474 bp长度的细...
Effect of Peripheral Administration of Kisspeptin-10 on Dynamic LH Secretion in Prepubertal Ewes
Kisspeptin-10 LH Prepubertal Ewes
2016/5/10
The aim of the present study was to clarify the effect of kisspeptin-10 on LH secretion in prepubertal ewes. In experiment 1, prepubertal ewes fitted with indwelling jugular catheters were randomly as...
An Antisense Oligodeoxynucleotide to the LH Receptor Attenuates FSH-induced Oocyte Maturation in Mice
Cumulus-enclosed Oocyte LH Receptor Oligodeoxynucleotide Mouse
2016/4/28
It has been recently shown that expression of the LH receptor (LHR) in cumulus cells is related with FSH-induced meiotic resumption of mouse cumulus enclosed oocytes (CEOs). However, to date, it is st...
摘要采用相对定量反转录多聚酶链式反应(RT-PCR)的方法,以β-actin为内标,测定绍鸭排卵前卵泡F1、F3、F5及大白卵泡(LWF)颗粒层与膜层中GH受体(GHR)、IGF-I型受体(IGF-IR)和 FSH受体(FSHR)、LH受体(LHR)mRNA表达水平,以分析生长轴和生殖轴激素对绍鸭卵泡发育的协同调节作用。结果表明:在所测定的各级卵泡中,GHR mRNA在膜层的表达水平均显著高于颗粒...
中药固真方对老年鼠睾丸间质细胞(Leydig细胞)LH受体的下行调节
间质细胞
2008/1/5
摘要 中药固真方是由首乌、苁蓉等六味中药组成的复方,具有补肾益精、延缓衰老及调整下丘脑-垂体-性腺-胸腺轴的功能,实验还证明它能显著增加老年大鼠大脑皮质和海马组织中的肾上腺素能受体(α_1和β)、多巴胺受体、胆碱能受体和γ-氨基丁酸受体。本文比较了
猪LHβ亚基基因的单核苷酸多态性研究
LHβ基因 SNPs 内含子 SSCP
2007/12/29
本研究对猪LHβ亚基基因除上游调控区以外的序列进行了SSCP分析,结果发现在该基因的3个外显子和3′调控区内没有任何SNPs位点,仅在内含子1的1367位点和内含子2的1823位点发现2个多态性位点,并对这两个位点在二花脸、约克夏和长白猪3个群体中的基因频率进行了初步分析。
Production of Biological Active Single Chain Bovine LH and FSH
Tethered-bLH and -FSH Biological Activity
2016/4/13
Luteinizing hormone as other glycoprotein hormones is characterized by a heterodimeric structure composed a common a-subunit noncovalently linked to a specific b-subunit. The correct conformation of t...