搜索结果: 1-9 共查到“生物学 StAR”相关记录9条 . 查询时间(0.125 秒)
当前,单链DNA序列的体外获取严重依赖于固相化学合成,其技术上已非常成熟,但依然具有明显的局限性。比如,化学合成DNA的产率随着序列长度的增加而显著降低,当超过200个碱基时,化学合成得到的DNA不仅产率/量低下,而且成本较高。换言之,化学合成长单链DNA的性价比很低,该缺点严重限制了很多基于长单链DNA序列的下游生物技术的应用,如长单链DNA模板介导的基因敲入、基于长单链DNA锁式探针的长程测序...
猪StAR基因启动子区克隆及其转录活性研究
StAR基因 启动子 荧光素酶活性 报告基因
2018/7/26
旨在通过分析猪StAR基因启动子活性区域,探究猪StAR基因的转录调控机制,从育种学角度为提高猪繁殖力提供新思路。本研究根据Ensembl数据库已公布的猪StAR基因的5'侧翼区序列,利用在线预测软件对该基因启动子区序列信息进行分析,以大白猪基因组DNA为模板,利用特异性引物,进行PCR扩增、测序,进而构建启动子区不同缺失片段的pGL3-StAR双荧光素酶表达载体,转染293T细胞并进行活性检测。...
A ‘STAR’ is born: Engineers devise genetic 'on' switch
STAR is born Engineers devise genetic switch
2015/3/4
All life processes depend on genes turning on and off. Cornell scientists have created a new “on” switch to control gene expression – a breakthrough that could revolutionize genetic engineering.Synthe...
Virus fingered as top suspect in West Coast sea star wasting disease(图)
Virus wasting disease
2014/12/1
The following is part twelve in a series on the NSF-NIH-USDA Ecology and Evolution of Infectious Disease (EEID) Program. See parts: one, two, three, four, five, six, seven, eight, nine, 10 and 11.
Mi...
Since 2013, millions of sea stars native to the Pacific coast of North America from Baja California to southern Alaska have succumbed to a mysterious wasting disease in which their limbs pull away fro...
Why sibling stars look alike: Early, fast mixing in star-birth clouds(图)
star-birth clouds sibling stars look alike
2014/9/12
Stars are made mostly of hydrogen and helium, but they also contain trace amounts of other elements, such as carbon, oxygen, iron, and even more exotic substances. By carefully measuring the wavelengt...
Species tree inference by the STAR method, and generalizations
Species tree inference STAR method generalizations Populations and Evolution
2012/5/2
The multispecies coalescent model describes the generation of gene trees from a rooted metric species tree, and thus provides a framework for the inference of species trees from sampled gene trees. We...
HCG通过StAR蛋白快速调节睾酮的合成
HCG 睾丸间质细胞 睾酮 类固醇合成快速调节蛋白
2013/12/11
以2~3周龄长白仔猪睾丸间质细胞为材料研究HCG对睾酮合成的快速调节作用。结果表明:(1)在2 h内,对照组间质细胞分泌睾酮的量为(3.25±0.28) ng/mL,而HCG(浓度为50 IU/mL)处理组睾酮的浓度为(8.79±054)ng/mL,增幅为4.54 ng/mL,二者差异极显著(P<0.01);(2) 在2 h内,对照组细胞内cAMP的浓度为(13.13±1.12 ) pmol/m...