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Towards the isolation of esterase and amidase catalytic antibodies: Examination of germinal center diversity by cloning and sequence analysis of genes encoding monoclonal antibodies elicited by a transition state analogue
Esterase catalytic antibodies amidase amide ester hydrolysis reaction hybrid cells B cells the TSA induced antibodies genes cells cell lines
2014/12/11
In this study, a transition state analogue (TSA) approach was employed for the isolation of catalytic antibodies with esterase or amidase activity. The TSA used in this study, phosphonamidate, mimics ...
Cloning and Sequence Analysis of Glyceraldehyde-3-Phosphate Dehydrogenase Gene in Yak
Yak Glyceraldehyde-3-Phosphate Dehydrogenase gene Cloning Housekeeping Gene
2016/4/28
In order to study the biological function of gapdh gene in yak, and prove whether the gapdh gene was a useful intra-reference gene that can be given an important role in molecular biology research of ...
摘要
PPAR基因是近年发现的与脂类代谢有重要关联的核受体基因。本项研究参考鸡、人类、啮齿类等动物的PPAR基因序列，用RT-PCR方法首次获得了鹅PPARα和PPARγ基因的cDNA序列，2个基因CDS长度分别为1407bp和1428bp。鹅与鸡、人、鼠等5种动物PPARα基因、PPARγ基因CDS序列同源性分别为87.43%、92.00%，氨基酸序列同源性分别为93.38%、96.95%。进...
蚕豆豆类胰岛素基因的cDNA克隆及序列分析cDNA Cloning and Sequence Analysis of Leginsulin Gene in Broad Bean (Vicia faba)
蚕豆 豆类胰岛素基因 cDNA序列 RT-PCR
2007/12/30
摘要为探明豆科植物中豆类胰岛素基因的结构特征与进化关系，在已获得大豆豆类胰岛素基因的基础上，以蚕豆种子胚根mRNA为材料，采用RT-PCR技术，克隆了蚕豆豆类胰岛素基因的cDNA序列，编码的前体多肽包括信号肽、成熟型豆类胰岛素及另一多肽的45个氨基酸残基。DNA序列分析表明，克隆片段与大豆和豌豆的同源性分别为62.5%和58.7%。在氨基酸水平上分别具有44.2%和43.6%的同源性，其中存在着高...
期刊信息
篇名
Proviral Genomic Sequence Analysis of Chinese Donkey Leukocyte Attenuated Equine Infectious Anemia Virus Vaccine and its Parental Virus Strain Liaoning
语种
中文
撰写或编译
作者
L.Wang,G.Z.Tong,H.Q.Liu,Z...
Fractals in DNA sequence analysis
2007/7/28
期刊信息
篇名
Fractals in DNA sequence analysis
语种
英文
撰写或编译
撰写
作者
Yu Zu-Guo,Vo Anh,Gong Zhi-Ming,Long Shun-Chao
第一作者单位
刊物名称
Chin. Phys.
页面
11(2002) 1313-1318
出版日期
2002年
12月
日
文章标识(ISSN)
相关项目
非线性科学方法在生命序列与金...
Chromosomal Localization of Korean Cattle (Hanwoo) BAC Clones via BAC end Sequence Analysis
Bovine BAC Library Pooling System BAC End Sequence Comparative Mapping Chromosomal Localization
2016/4/25
In this study, a Korean native cattle strain (Hanwoo) evidencing high performance in terms of both meat quality and quantity was employed in the generation of 150,000 BAC clones with an average insert...
Isolation of DNA for Sequence Analysis from Herbarium Material of Some Lichen Specimens
DNA isolation herbarium material lichen sequence analysis
2010/3/19
An improved protocol for the isolation of DNA from herbarium material of some lichen specimens is described. The isolated DNA is suitable for PCR reactions for DNA sequence analysis. The hexadecyltrim...
Sequence analysis of the gliding protein Gli349 in Mycoplasma mobile
repeat sequence Gli349 motility YGF motif Mycoplasma mobile sequence analysis
2010/12/2
The motile mechanism of Mycoplasma mobile remains unknown but is believed to differ from any previously identified mechanism in bacteria. Gli349 of M. mobile is known to be responsible for both adhesi...