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The stability of T7 RNA polymerase elongation complexes and promoter function analysis by in vitro selection
Forward translocation model polymerase complex stability DNA structure
2014/12/11
The forward translocation model (paused or stalled polymerase moves forward in the absence of transcription, dissociation of the complex happens when hybrid is too short to stabilize the complex) is t...
Functional tests of structural models for abortive cycling in T7 RNA polymerase
Pure sciences Biological sciences Abortive cycling RNA polymerase Transcription
2014/12/10
Transcription initiates when the enzyme binds to the promoter region and melts open an initial transcribing bubble that extends from position -4 to +4. The initiation phase continues until the enzyme ...
Detection of the Paulownia Witches＇ Broom Mycoplasmalike Organism by Polymerase Chain Reaction
Paulownia witches’brom Mycoplasmalike organism polymerase chain reaction Pathogen diagnosis
2009/9/23
A polymerase chain reaction (PCR)method with high sensitivity and specificity has been developed
with a pair of oligonucleotide primers designed and synthesized according to the DNA sequence specific...