搜索结果: 1-6 共查到“动物遗传学 SRY”相关记录6条 . 查询时间(0.062 秒)
Detection of the SRY Transcript and Protein in Bovine Ejaculated Spermatozoa
SRY Gene Bovine Spermatozoa In situ Hybridization Bovine
2016/5/9
The sex-determining region on the Y (SRY) gene is important in mammalian sex determination and differentiation. We report a study of the abundance of SRY gene products in bovine ejaculate. RT-PCR expe...
Amplification of Porcine SRY Gene for Sex Determination
SRY Gene Sex Determination Sperm Sorting Duplex PCR
2016/4/29
The separation of X and Y chromosome-bearing sperm is of use in many aspects of livestock maintenance. In this study, we sought to determine the difference in DNA content between X- and Y-bearing sper...
Discrimination of Korean Cattle (Hanwoo) Using DNA Markers Derived from SNPs in Bovine Mitochondrial and SRY Genes
Hanwoo Bos taurus Bos indicusx Bos indicus PCR-RFLP SRY ND4 ND5
2016/4/27
In order to distinguish Korean cattle (Hanwoo) beef from the imported beef from Australia in Korean markets, DNA markers based on PCR-RFLP from mitochondrial genes and SRY gene were applied. A total o...
利用SRY基因和微卫星标记鉴定反刍动物性别
SRY基因 微卫星标记 性别鉴定
2007/12/20
以反刍动物为研究对象，应用多重PCR技术扩增绵羊基因组中X、Y染色体上的4个微卫星标记和SRY基因， 根据基因型进行性别鉴定，试图通过一次DNA扩增同时提供性别鉴定和基因分型的信息。结果表明所设计SRY基因的引物具有高度特异性，是性别鉴定的主要依据，而Y染色体上的MCM158、MAF45两标记由于特异性不好，因此不适用于性别鉴定，对于X染色体上所选的两标记MILVET09和AE25只能进一步验...
摘要：采用人SRY基因的一段保守序列的引物，通过PCR在雄性赤麂中扩增出了赤麂SRY 基因的特异片段,通过DNA斑点杂交证实其扩增产物与人SRY基因有着较高的同源性。将PCR产物克隆到载体pGEM® T Vector中,转化DH5α菌,经与人SRY基因探针进行菌落杂交筛选出赤麂SRY基因的阳性克隆,并对其进行了测序.将其序列与基因库中录入的所有偶蹄目动物的SRY基因序列进行同源性比较，用U...
A Y-linked SNP in SRY Gene Differentiates Chinese Indigenous Swamp Buffalo and Introduced River Buffalo
Swamp buffalo River buffalo SRY SNP Genetic diversity Conservation
2016/4/21
The complete coding region sequence of the SRY gene in Chinese swamp buffalo was determined by PCR product sequencing. Comparison of swamp and river buffalo SRY gene sequences revealed a single nucleo...