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In vivo and in vitro Cleavage of Glucoamylase-TNFα Fusion Protein Secreted from Aspergillus niger
filamentous fungi Aspergillus niger heterologous protein secretion
2009/3/19
The most common expression strategy for secreting heterologous proteins from filamentous fungus Aspergillus niger is based on fusion with glucoamylase gene which contains cleavage site for kexin prote...
Integrative Expression of Glucoamylase Gene in a Brewer’s Yeast Saccharomyces pastorianus Strain
glucoamylase brewer’s yeast diacetyl fermentation
2009/2/23
The recombinant brewer’s yeast Saccharomyces pastorianus strain was constructed by introducing the ilv2::GLA fragment released from pMGI6, carrying glucoamylase gene (GLA) and using the yeast α-acetol...
Site-Directed Mutagenesis of Tryptophan 622 of Thermoactinomyces vulgaris R-47 Glucoamylase:pH Optima and Activities of Five Mutants
glucoamylase optimal pH catalytic base
2008/4/20
In Aspergillus awamori glucoamylase, the optimal pH has been reported to increase to maintain activity by a mutation of Ser411 which forms a hydrogen-bond with a catalytic base (Fang and Ford, Protein...