搜索结果: 1-6 共查到“农学 PPV”相关记录6条 . 查询时间(0.046 秒)
Preliminary results of in vivo thermotherapy of plum, apricot and peach cultivars artificially infected with PPV-M and PPV-D strains of Plum pox virus
apricot peach plum
2015/1/28
The elimination of Plum pox virus (PPV) in different stone fruit cultivars was verified by the method of thermotherapy in vivo. Trees of two plum cultivars 萢鑑nsk?lepotica and 妚estka dom醕? apricot cult...
To the distribution of PPV-M strain in selected orchards of apricots and peaches in the Czech Republic
apricot peach orchards Plum pox virus
2015/1/21
The presence and distribution of PPV-M strain of Plum pox virus were investigated in selected orchards of apricots and peaches in the Czech Republic. PPV-M was found to be distributed in apricot and p...
为分析猪圆环病毒2型( PCV2) 与猪细小病毒 ( PPV) 体外共感染对猪外周血单个核细胞( PBMC) 细胞凋亡相关因子mRNA转录水平的影响，探讨PCV2和PPV共感染机制及宿主—病毒之间的作用关系，运用病毒滴度和相对荧光定量PCR技术，测定和分析PCV2和PPV感染PBMC后PCV2、PPV的病毒滴度含量及Bcl-2、FasL、p53、Caspase-8、PBR、TNF-α等的转录时相变...
将干扰素-γ、猪圆环病毒Ⅱ型ORF2主要抗原表位（47—85位AA）和猪细小病毒VP2基因克隆至pCI-neo真核载体中，构建重组质粒pCI-IFN-γ.ORF2.VP2。用脂质体介导法将其转染到MDBK细胞中，利用RTPCR和间接免疫荧光法检测产物的表达情况。将重组质粒pCI-IFN-γ.ORF2.VP2、pCI-ORF2.VP2、对照质粒pCI-neo、PBS、猪细小病毒灭活苗和猪圆环病毒亚...
猪细小病毒（PPV）SD1株NS1 基因的克隆与原核表达
猪细小病毒 NS1基因 克隆 原核表达
2009/6/5
[ 目的] 为建立猪细小病毒的快速诊断方法提供理论依据。[ 方法] 根据GenBank 上猪细小病毒基因组序列和原核表达质粒pET30a
( +) 多克隆位点序列设计1 对引物, 应用PCR 技术扩增出猪细小病毒SD1 株NS1 基因全序列, 对阳性重组质粒进行测序和同源性比较。
构建原核表达重组质粒pET30a/ NS1 , 并在大肠杆菌中进行诱导表达。[ 结果] 通过PCR 扩增获得2 2...
Detection and Characterization of Plum Pox Potyvirus（PPV）by DAS-ELISA and RT-PCR / RFLP Analysis in Turkey
Enzyme-linked immunosorbent assay plum pox potyvirus restriction analysis reverse transcription-polymerase chain reaction
2009/1/13
This study was conducted to determine the presence of plum pox virus (PPV) (family Potyviridae, genus Potyvirus) in different regions of Turkey and to characterize PPV isolates by serological and mol...