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Studies on Culture of Cells and Tissues of Crop Plants:III. Isolation of rice mesophyll protoplasts with a new cell wall lytic enzyme "funcelase"
Cell wall Cellulase "Onozuka" R-10 Funcelase Mesophyll Oryza sativa L
2009/8/6
Rice mesophyll protoplasts were isolated with ease and with high yield from expanded 2nd leaves, which were grown on agar plates under 23°C, 16.5 W/m2 for 25 days, by using a new cell wall lytic enzym...
Studies on Culture of Cells and Tissues of Crop Plants:I. Survey on enzymatic isolation and culture of rice leaf sheath protoplasts
BSA Cell activity Cellulase "Onozuka" R-10 Protoplasts MS-medium Rice leaf sheath
2009/8/6
To establish a method for isolation and culture of rice protoplasts, enzyme solution compositions, culture conditions, and the selection of cultivars and tissues as materials were examined. The result...
Morphological Study of Soybean Protoplasts in Early Electrofusion Process
Electrofusion Fusion process Soybean protoplast Ultrastructure
2009/8/6
The present study is intended to analyze the early response of protoplasts isolated from soybean suspension cells to electrofusion. It was observed by light microscopy that protoplasts formed a pearl ...
Studies on Cell Wall Regeneration and Cell Division in Soybean Protoplasts Using Fluorescence and Scanning Electron Microscopy
Calcofluor White Cell wall formation Cell division SEM Soybean protoplast
2009/8/5
Protoplasts were enzymatically isolated from soybean suspension cells and were cultured in a liquid medium. The reappearance of cell wall at the surface of naked protoplasts was studied by fluorescenc...
Ultrastructure of Electrofused Products from Pansy (Viola tricolor) Mesophyll and Wild Viola (V. patrinii) Petiole Callus Protoplasts
Electrofusion Pansy Protoplast Ultrastructure Wild viola
2009/8/5
Ultrastructural observations were undertaken to study the viability of fusion products by electrofusing the protoplasts from the mesophyll cells of pansy leaves and the cultured callus cells of wild v...
Ultrastructural Analysis of Cell Wall Formation and Cell Division of Soybean Protoplasts
Cell division Cell wall formation Protoplasts Soybean TEM Ultrastructure
2009/8/4
Protoplasts of soybean (Glycine max (L.) Merr.) were isolated from suspension cultures and morphological changes during culture were observed using transmission electron microscopy. The regeneration ...
Effects of Electro- and Chemical Fusion Treatment on Cell Wall Formation and Cell Division of Plant Protoplasts
Cell division Cell wall Fusion Protoplast
2009/7/31
The effects of electro- and chemical fusion procedures on cell wall formation and cell division of lettuce and soybean protoplasts were studied. Judging from Calcofluor White staining, cell wall forma...
An Efficient Method for Callus Formation of Protoplasts from Peanut (Arachis hypogaea L.) Leaf Mesophyll
Callus formation Mesophyll protoplast Peanut Protoplast culture
2009/7/30
High yields of viable protoplasts were obtained from leaf mesophyll of 10-day-old peanut seedlings (Arachis hypogaea L. var. Lokal). An enzyme solution containing 1% Cellulase YC, 2% Meicelase P-1, 0....
Evaluation of Preparation Methods for Scanning Electron Microscopic Observation of Plant Protoplasts
Dehydration Fixation method Petunia Protoplast Protoplast size Rice
2009/7/28
Preparation methods for scanning electron microscopy (SEM) were studied with petunia and rice protoplasts. Fixation schedules to sustain protoplast size during alcohol dehydration were examined. When ...
Ultrastructural Changes of Chloroplasts in Peanut Mesophyll Protoplasts Treated with Electric Fields
Chloroplast division Chloroplast fusion Electric fields Mesophyll protoplasts Peanut
2009/7/28
Ultrastructural changes in chloroplasts of peanut (Arachis hypogaea L. var. Lokal) seedling mesophyll cells and mesophyll protoplasts treated with electric fields were investigated. Generally, chloro...
Ultrastructural Studies on the Fusion Product between Protoplasts of Mung Bean and Adzuki Bean
Adzuki bean Chloroplast Fusion Mung bean Organelles Protoplast
2009/7/27
Mesophyll protoplasts of mung bean (Vigna radiata L. Wilczek) were electrofused with cell suspension-derived protoplasts of adzuki bean (V. angularis Ohwi & Ohashi) and subsequently cultured for seve...
Effects of Brassinosteroid on Cell Division and Colony Formation of Chinese Cabbage Mesophyll Protoplasts
Brassinosteroid Cell division Chinese cabbage Colony formation Epibrassinolide Mesophyll protoplast
2009/7/24
Effects of epibrassinolide (EBR), one of the brassinosteroids (BRs), on culture of Chinese cabbage mesophyll protoplasts were researched. The addition of EBR to the liquid culture medium increased the...
Isolation and regeneration of protoplasts from the mycelium of Fusarium pallidoroseum – a potential biocontrol agent of water hyacinth [Eichhornia crassipes（Mart）Solms]
Protoplast isolation regeneration
2009/2/19
A protocol for isolation of protoplast from the mycelium of Fusarium pallidoroseum, a potential biocontrol agent of water hyacinth, was standardized. Stable and more medium sized protoplasts were obta...
灵芝原生质体的分离及其转化Preparation and Regeneration of the Protoplasts from Ganoderma lucidum and Their Transformation
灵芝 原生质体 再生 转化
2007/12/7
摘要对灵芝(赤芝)进行了原生质体的形成和再生实验,研究了渗透压稳定剂、菌龄、酶解条件对原生质体分离的影响,以及渗透压稳定剂、培养基对原生质体再生的影响;另外还尝试用电穿孔法在灵芝原生质体中进行报告基因(reportgene)β-gal的转化,结果β-gal在菌丝体中有表达。
Abstract:The experiments of protoplasts preparation from Ling...