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Peroxisome proliferator activated receptor ligands affect porcine endometrial steroids production during the estrous cycle and early pregnancy:an in vitro study
PPAR progesterone estradiol luteal phase periimplantation
2016/9/1
In the present study, we investigated the effect of PPAR ligands on progesterone (P4) and 17β-estradiol (E2) secretion, as well as 3β-hydroxysteroid dehydrogenase/Δ(5)-Δ(4) isomerase (3β-HSD) mRNA exp...
Expression analysis of the porcine Homeobox A11 gene and its association with litter size in Large White pigs
endometrium HOXA11 qPCR single nucleotide polymorphism litter size
2016/6/3
The homeobox A11 (HOXA11) gene, a well-known transcriptional regulator, plays a critical role in normal development of the uterus. In this study, the porcine HOXA11 gene, including its promoter region...
The role of nitric oxide synthase isoforms in aged porcine oocytes
nitric oxide L-NAME aminoguanidine oocyte ageing pig
2015/6/24
In the sphere of reproductive biotechnologies, the demand for sufficient numbers of high-quality oocytes is still increasing. In some cases, this obstacle is overcome by in vitro prolonged cultivation...
Expression and localization of nitric oxide synthase isoforms during porcine oocyte growth and acquisition of meiotic competence
pig oocyte growth meiotic competence
2015/6/8
Reproduction biotechnologies depend on the use of fully meiotically competent oocytes. Growing oocytes without full meiotic competence are an interesting potential source due to their quantity, but th...
Embryo Aggregation Promotes Derivation Efficiency of Outgrowths from Porcine Blastocysts
In vitro-produced Embryos Embryo Quality Derivation Efficiency Pig
2016/5/19
Porcine embryonic stem cells (pESCs) have become an advantageous experimental tool for developing therapeutic applications and producing transgenic animals. However, despite numerous reports of putati...
Genotyping of porcine teschoviruses isolated from 1960 to 1980 in the former Czechoslovakia and new Porcine teschovirus isolates obtained from piglets with diarrhoea
fecal samples reverse transcription polymerase chain reaction
2015/4/1
The porcine enteroviruses (family Picornaviridae) were described as causative agents of neurological disorders known as Teschen/Talfan disease, reproductive failure, dermal lesions, enteric disease an...
Serum deprivation affects secretory activity of cultured porcine ovarian follicles and granulosa cells and their response to hormones
stress malnutrition IGF-I
2015/4/1
The aim of the present study is to understand the hormonal mechanisms of the effect of malnutrition on ovarian follicle functions. For this purpose, we examined the effect of malnutrition/serum depriv...
Production of Mutated Porcine Embryos Using Zinc Finger Nucleases and a Reporter-based Cell Enrichment System
Knockout Pig Reporter Based Enrichment Somatic Cell Nuclear Transfer Zinc Finger Nuclease
2016/5/16
To facilitate the construction of genetically-modified pigs, we produced cloned embryos derived from porcine fibroblasts transfected with a pair of engineered zinc finger nuclease (ZFN) plasmids to cr...
Effect of Dipeptides on In vitro Maturation, Fertilization and Subsequent Embryonic Development of Porcine Oocytes
Ammonia Accumulation Dipeptides Incorporation and Oxidation Porcine Embryos
2016/5/11
The effects of amino acids and dipeptides on in vitro production of porcine embryos and accumulation of ammonia in culture medium during developmental stages were examined in this study. The maturatio...
Pro-apoptotic Effect of Pifithrin-α on Preimplantation Porcine In vitro Fertilized Embryo Development
Apoptosis In vitro Fertilization Pifithrin-α Blastocyst Preimplantation Embryo
2016/5/11
The aim of this study was to investigate the impact of a reported p53 inhibitor, pifithrin-α (PFT-α), on preimplantation porcine in vitro fertilized (IVF) embryo development in culture. Treatment of P...
Effect of the Addition of β-Hydroxybutyrate to Chemically Defined Maturation Medium on the Nuclear Maturation, Sperm Penetration and Embryonic Development of Porcine Oocytes In vitro
Porcine oocytes β-Hydroxybutyrate Nuclear Maturation Embryonic Development
2016/5/5
We investigated the effects of various concentrations of β-hydroxybutyrate (BHB, 0, 0.1, 1 and 10 mM), a ketone body, added to chemically-defined maturation medium with or without energy substrates (g...
Expression Analysis of miRNAs in Porcine Fetal Skeletal Muscle on Days 65 and 90 of Gestation
Pig MicroRNA Expression Skeletal Muscle
2016/4/28
MiRNAs (microRNAs) are a class of small non-coding RNA molecules of ~21 nucleotides that down- regulate the expression of target genes at post-transcriptional level. In this study, we first accomplish...
Improvement of developmental competence of aged porcine oocytes by means of the synergistic effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF)
epidermal growth factor insulin-like growth factor 1 oocyte ageing pig
2015/6/1
There is a constant increase in demand for large numbers of high-quality oocytes of domestic mammals for various reproductive biotechnologies. Prolonged in vitro culture represents one of the many pos...
The Effect of Spray-dried Porcine Plasma and Tryptophan on Feed Intake and Performance of Weaning Piglets
Weaning Piglet Tryptophan Feed Intake Performance
2016/4/18
There were three trials involved in this experiment. All piglets in Trial 1 were randomly distributed into the following 4 treatments. Treatment 1. Corn-soybean diet with 5% SDPP. The tryptophan level...
Effects of Dietary Zinc on Performance and Immune Response of Growing Pigs Inoculated with Porcine Reproductive and Respiratory Syndrome Virus and Mycoplasma hyopneumoniae
Pigs PRRSv Zinc Growth Immune Response
2016/4/18
The objective of this study was to determine the effects of dietary Zn level on performance, serum Zn concentrations, alkaline phosphatase activity (ALP), and immune response of pigs inoculated with P...