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研究钙离子载体A23187诱发精子顶体反应率、顶体酶总活性与精液参数及体外受精(IVF)结局的关系，初步探讨钙离子载体A23187诱发精子顶体反应、顶体酶总活性预测男性生育力及IVF结局的价值，比较这两种检验方法的临床应用价值。方法 应用钙离子载体A23187在体外诱发精子顶体反应，采用改良Kennedy法检测精子顶体酶总活性。研究IVF-ET治疗150周期患者及各亚组中男方的精子顶体反应率、顶体...
A23187联合IFN-γ诱导人外周血单个核细胞生成树突状细胞
钙离子载体 γ- 干扰素 树突状细胞 外周血单个核细胞
2012/11/1
研究钙离子载体(calcium ionophore, CI)A23187 联合γ- 干扰素(IFN-γ) 诱导健康人外周血单个核细胞(PBMNC) 生成树突状细胞(DC), 探索DC 扩增的新方法。方法:分离健康人PBMNC, 分别加入GM-CSF +IL-4, A23187, A23187+IFN-γ。体外培养72 h 后, 分别于光镜、电镜下观察细胞的形态, 流式细胞仪检测细胞表面标志, M ...
Analysis of human sperm function following exposure to the ionophore A23187. Comparison of normospermic and oligozoospermic men
A23187 sperm motility fertilization ohgozoospermia
2009/2/20
Time exposure photomicrography and interspecific in vitro fertilization techniques have been used to compare the responses to the divalent cation ionophore A23187 of spermatozoa from normal fertile an...
Involvement of reactive oxygen species in human sperm arcosome reaction induced by A23187, lysophosphatidylcholine, and biological fluid ultrafiltrates
Hydrogen peroxide catalase superoxide dismutase chemiluminescence
2009/2/18
Although recent evidence indicated that the production of reactive oxygen species (ROS) by human spermatozoa may be involved in the regulation of capacitation, very little is known about the role ...
Analysis of the responses of human spermatozoa to A23187 employing a novel technique for assessing the acrosome reaction
A231 87 acrosome reaction oocyte fusion human
2009/2/12
Protocols for the use of A23187 in assessing the ability of human spermatozoa to acrosome-react and exhibit sperm-oocyte fusion have been developed and the results compared in two independent cohort...