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CRISPR-Cas基因编辑技术是本世纪颇具影响力的颠覆性创新技术，其发明者荣获2020年诺贝尔化学奖。当前已开发CRISPR-Cas基因编辑工具多依赖于靶点DNA双链切割，并需借助宿主自身的同源重组或者非同源末端连接DNA修复系统实现基因编辑，脱靶效应和编辑效率低是其瓶颈问题，阻碍了该技术在人类疾病治疗等关键领域的应用。开发更高效精准的无需DNA双链断裂的基因编辑工具是该领域亟待解决的科学问题。
Effect of ferrule on fracture on fracture resistance of cast post and core ?In vitro study
fracture resistance cast post
2010/11/3
Effect of ferrule on fracture on fracture resistance of cast post and core ?In vitro study.
Accuracy of fit of cast clasp designed with conventional wax pattern and light cured patterns - A comparative in vitro study
Accuracy wax pattern light cured patterns
2010/11/3
Accuracy of fit of cast clasp designed with conventional wax pattern and light cured patterns - A comparative in vitro study.
Retention of Fiber and Cast Posts with Different Lengths:A Comparative Study
Post and Core Technique Dental Restoration,Permanent Lightpost Dental
2009/12/9
Objective:
There is no definitive data on the strength of glass fiber and cast posts with different length. This in vitro study was designed to investigate and compare the effect of length on the ret...
The influence of mold temperature on the fit of cast crowns with commercially pure titanium
Marginal adaptation Crowns Titanium Dental casting investment
2009/10/19
Commercially pure titanium (CP Ti) has been widely applied to fabricate cast devices because of its favorable properties. However, the mold temperature recommended for the manufacture of casts has bee...
Comparison of the time required for removal of intraradicular cast posts using two Brazilian ultrasound devices
Post and core technique Endodontics Ultrasonics
2009/10/16
The aim of this in vitro study was to compare the time required for removal of intraradicular cast posts cemented with zinc phosphate (ZF) or glass ionomer cement (GIC), using two Brazilian ultrasound...
Effects of Welding Pulse Energy and Fluoride Ion on the Cracking Susceptibility and Fatigue Behavior of Nd:YAG Laser-welded Cast Titanium Joints
Welding pulse energy Fluoride Mechanical property
2009/9/15
In this study, the cracking susceptibility and fatigue behavior of Nd:YAG laser-welded cast Ti joints (welding pulse energy: 11, 15, and 18 J) in fluoride-containing (0 and 0.5% NaF) artificial saliva...
Experimental Binder-free Investments Reused to Cast Dental Precious Alloys
Investment Cast Reuse
2009/9/14
This study aimed to develop reusable dental investments. SiO2 and MgO were selected as refractory materials to prepare three types of investment (coded as 60S-40M, 80S-20M, 100S) with 40, 20, and 0 wt...
Anodic polarization tests were performed in 0.9% NaCl and 1% lactic acid solutions to characterize the relationship between the corrosion behavior and the microstructures of cast Ti-Ag (5-40% Ag) allo...
Fracture Resistance of Nd:YAG Laser-welded Cast Titanium Joints with Various Clinical Thicknesses and Welding Pulse Energies
Fracture resistance Thickness Welding pulse energy
2009/9/14
The purpose of this study was to evaluate the fracture resistance of Nd:YAG laser-welded cast titanium (Ti) joints with various clinical thicknesses and welding pulse energies. A four-point bending te...
Recording Tooth Movement with the Sectioned Oriented Cast
Recording Tooth Movement Sectioned Oriented Cast
2009/3/17
Recording Tooth Movement with the Sectioned Oriented Cast.
Recording the Frankfort-Mandibular-Plane Angle Using Oriented Mandibular Radiogram and Sectioned Oriented Cast
Frankfort-Mandibular-Plane Angle Oriented Mandibular Radiogram Sectioned Oriented Cast
2009/3/17
Recording the Frankfort-Mandibular-Plane Angle Using Oriented Mandibular Radiogram and Sectioned Oriented Cast.
Isolated Hematuria in SLE Patients and its Association with Proteinuria, Uri-nary Cast and SLE Disease Activity
Isolated hematuria systemic lupus erythematosus SLE disease activity
2009/12/16
Isolated hematuria and its association with proteinuria, and urinary cast and systemic lupus erythematosus (SLE) disease activity, and decision for renal biopsy is a dilemma for physician in SLE patie...