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Comparison of the penetration ability of human spermatozoa into bovine cervical mucus and zona-free hamster eggs
penetration ability human spermatozoa bovine cervical mucus zona-free hamster eggs
2009/2/20
In vitro bovine cervical mucus (BCM) penetration tests, sperm penetration assays (SPA) using zona-free hamster eggs, and routine semen analyses were performed on a total of 136 freshly collected sem...
Effects of white blood cells on the in vitro penetration of zona-free hamster eggs by human spermatozoa
white blood cells zona-free hamster eggs human spermatozoa
2009/2/20
The presence of white blood cells in semen has been associated with male infertility. Previous studies indicate that pyospermia occurs in conjunction with decreases in sperm motility, number of no...
Comparison of glycerol and a zwitter ion buffer system as cryoprotective media for human spermatozoa. Effect on motility, penetration of zona-free hamster oocytes, and acrosin/proacrosin
spermatozoa human cryopreservation
2009/2/20
This study compared the cryoprotective effect of glycerol with that of a zwitter ion buffer system (TESTCY). Spermatozoa that are cryopreserved in the presence of glycerol possess a somewhat highe...
The Effect of Inhibitors of Trypsin and Phospholipase A2 on the Penetration of Zona Pellucida-free Hamster Eggs by Acrosome-reacted Hamster Sperm
Trypsin Phospholipase A2 Penetration Zona Pellucida-free Hamster Eggs
2009/2/19
The involvement of a trypsin-like enzyme and a phospholipase A2 in hamster sperm-egg fusion was investigated. Previously acrosome-reacted sperm and zona pellucida-free eggs were incubated in a low...
Ultrastructural Observations on the Penetration of Human Sperm into the Zona Pellucida of the Human Egg In Vitro
ultrastructure zona pellucida human ova acrosome reaction
2009/2/19
Sperm penetration into the zona of 46 human eggs was examined by electron microscopy. Preovulatory oocytes were aspirated at laparoscopy and inseminated by methods which produced normal pregnancie...
Evidence for the role of heterotrimeric guanine nucleotide-binding regulatory proteins in the regulation of the mouse sperm adenylyl cyclase by the egg's zona pellucida
Pertussis toxin acrosome reaction mastoparan cyclic
2009/2/18
Sperm acrosomal exocytosis is the result of a complex set of signal transduction pathways activated physiologically by the egg's extracellular matrix, the zona pellucida. In the mouse, the zona pe...
Sequence divergence within the sperm-specific polypeptide TCTE1 is correlated with species-specific differences in sperm binding to zona-intact eggs
Sperm egg binding zona pellucida evolution fitness
2009/2/17
The T-complex-associated testes-expressed (TCTE1) gene encodes a novel sperm cell-specific polypeptide (TCTE1) that is conserved across vertebrate species. TCTE1 is absolutely required for fertili...
Hyaluronic acid enhances the zona pellucida-induced acrosome reaction of macaque sperm
Macaca fascicularis semen capacitation fertilization
2009/2/17
Hyaluronic acid (HA) not only surrounds the zona pellucida as part of the cumulus matrix but also is present throughout the zona pellucida and the perivitelline space of many mammalian oocytes. Ho...
Use of peanut agglutinin to assess the acrosomal status and the zona pellucida-induced acrosome reaction in stallion spermatozoa
FITC-PNA hemizona acrosome reaction
2009/2/17
Peanut agglutinin (PNA) was used to assess the sperm acrosomal status and the acrosome reaction during gamete interaction in the equine species. PNA exclusively binds to the outer acrosomal membra...
Anti-bull sperm monoclonal antibodies：II. Binding changes during capacitation and influence on sperm-zona interactions in vitro
Bull sperm sperm-zona binding capacitation
2009/2/16
Anti-bull sperm monoclonal antibodies (mAbs), generated against intra-acrosomal and surface antigens, were evaluated for their functional significance. In experiment I, the influence of mAbs on the bo...
Compromised sperm protein phosphorylation after capacitation, swim-up, and zona pellucida exposure in teratospermic domestic cats
Cat sperm teratospermia tyrosine phosphorylation zona pellucida
2009/2/16
Tyrosine phosphorylated proteins recently have been found in mouse and human spermatozoa. Our objectives were to (1) determine if domestic cat spermatozoa also express tyrosine phosphorylated pro...
Effects of glucose and other energy substrates on the hyperactivated motility of macaque sperm and the zona pellucida-induced acrosome reaction
glucose hyperactivated motility macaque sperm
2009/2/13
Energy sources in sperm capacitation media have various effects on mammalian sperm and are required for stimulation of hyperactivated motility and/or acrosome reactions in some species. The presen...
Expression of mannose-binding sites on human spermatozoa and their role in sperm-zona pellucida binding
mannose-binding sites human spermatozoa sperm-zona pellucida binding
2009/2/13
A D-mannosylated albumin (DMA) neoglycoprotein was assessed to validate experimentally a probe capable of detecting mannose-binding sperm receptors involved in human sperm-egg interaction. DMA spe...
A morphometric comparison of the nuclear morphology of fresh and frozen-thawed human zona-bound and unbound sperm
Sperm nucleus computer-assisted morphometry zona binding cryopreservation DNA
2009/2/12
The role of sperm nuclear morphology in zona binding and the effect of cryopreservation on sperm nuclear morphology have been investigated using objective criteria. The nuclear dimensions of fresh a...
Zona pellucida piercing enhances zona penetration by spermatozoa from normospermic and teratospermic domestic cats
Felid micromanipulation sperm teratospermia
2009/2/12
Poor quality ejaculates appear associated with reduced zona penetration and poor fertilization in vitro in some felid species or populations. Even morphologically normal sperm from these teratospe...