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The Effect of Inhibitors of Trypsin and Phospholipase A2 on the Penetration of Zona Pellucida-free Hamster Eggs by Acrosome-reacted Hamster Sperm
Trypsin Phospholipase A2 Penetration Zona Pellucida-free Hamster Eggs
2009/2/19
The involvement of a trypsin-like enzyme and a phospholipase A2 in hamster sperm-egg fusion was investigated. Previously acrosome-reacted sperm and zona pellucida-free eggs were incubated in a low...
Ultrastructural Observations on the Penetration of Human Sperm into the Zona Pellucida of the Human Egg In Vitro
ultrastructure zona pellucida human ova acrosome reaction
2009/2/19
Sperm penetration into the zona of 46 human eggs was examined by electron microscopy. Preovulatory oocytes were aspirated at laparoscopy and inseminated by methods which produced normal pregnancie...
Evidence for the role of heterotrimeric guanine nucleotide-binding regulatory proteins in the regulation of the mouse sperm adenylyl cyclase by the egg's zona pellucida
Pertussis toxin acrosome reaction mastoparan cyclic
2009/2/18
Sperm acrosomal exocytosis is the result of a complex set of signal transduction pathways activated physiologically by the egg's extracellular matrix, the zona pellucida. In the mouse, the zona pe...
Hyaluronic acid enhances the zona pellucida-induced acrosome reaction of macaque sperm
Macaca fascicularis semen capacitation fertilization
2009/2/17
Hyaluronic acid (HA) not only surrounds the zona pellucida as part of the cumulus matrix but also is present throughout the zona pellucida and the perivitelline space of many mammalian oocytes. Ho...
Use of peanut agglutinin to assess the acrosomal status and the zona pellucida-induced acrosome reaction in stallion spermatozoa
FITC-PNA hemizona acrosome reaction
2009/2/17
Peanut agglutinin (PNA) was used to assess the sperm acrosomal status and the acrosome reaction during gamete interaction in the equine species. PNA exclusively binds to the outer acrosomal membra...
Compromised sperm protein phosphorylation after capacitation, swim-up, and zona pellucida exposure in teratospermic domestic cats
Cat sperm teratospermia tyrosine phosphorylation zona pellucida
2009/2/16
Tyrosine phosphorylated proteins recently have been found in mouse and human spermatozoa. Our objectives were to (1) determine if domestic cat spermatozoa also express tyrosine phosphorylated pro...
Effects of glucose and other energy substrates on the hyperactivated motility of macaque sperm and the zona pellucida-induced acrosome reaction
glucose hyperactivated motility macaque sperm
2009/2/13
Energy sources in sperm capacitation media have various effects on mammalian sperm and are required for stimulation of hyperactivated motility and/or acrosome reactions in some species. The presen...
Expression of mannose-binding sites on human spermatozoa and their role in sperm-zona pellucida binding
mannose-binding sites human spermatozoa sperm-zona pellucida binding
2009/2/13
A D-mannosylated albumin (DMA) neoglycoprotein was assessed to validate experimentally a probe capable of detecting mannose-binding sperm receptors involved in human sperm-egg interaction. DMA spe...
Zona pellucida piercing enhances zona penetration by spermatozoa from normospermic and teratospermic domestic cats
Felid micromanipulation sperm teratospermia
2009/2/12
Poor quality ejaculates appear associated with reduced zona penetration and poor fertilization in vitro in some felid species or populations. Even morphologically normal sperm from these teratospe...
Effect of fucoidin on human sperm-zona pellucida interactions
human sperm-zona binding fucoidin acrosome
2009/2/11
The authors recently reported that fucoidin (a polymer of predominantly L-fucose sulfate) produced a strong, significant, and dose-dependent inhibition of sperm-zona binding under hemizona assay c...
Sperm-zona pellucida interaction in cynomolgus and rhesus macaques
Sperm-zona pellucida interaction cynomolgus rhesus macaques
2009/2/11
These experiments were carried out to establish and validate an in vitro system for studying macaque sperm-zona pellucida interaction. Sperm of rhesus and cynomolgus macaques were capacitated in vit...
Functional aspects of human sperm binding to the zona pellucida using the hemizona assay
Human zona pellucida hyperactivation infertility
2009/2/7
The hemizona assay (HZA) has facilitated investigations of sperm function in relation to zona pellucida binding. In this study, the authors examined: 1) the association between hyperactivated sperm ...
Cryopreservation of a small number of fresh human testicular spermatozoa and testicular spermatozoa cultured in vitro for 3 days in an empty zona pellucida
Cryopreservation human testicular spermatozoa testicular spermatozoa cultured vitro zona
2008/12/25
It is known that the motility of human testicular sperm can be improved when they are cultured in vitro for a few days. The purpose of this study was to determine whether it is better to freeze huma...
Acrosome Reaction Induced by Recombinant Human Zona Pellucida 3 Peptides rhuZP3a22176 and rhuZP3b177348 and Their Mechanism
Human sperm pertussis toxin intracellular calcium G protein calcium channel chlortetracycline
2008/12/24
To explore the biological characteristics of the recombinant zona pellucida 3 (ZP3) peptides of rhuZP3a22176 and rhuZP3b177348, we examined whether rhuZP3a22176 or rhuZP3b177348 trigger the acros...
Examination of the Binding Ability of Bovine Spermatozoa to the Zona Pellucida as an Indicator of Fertility
Fertilization oocyte accessory cattle nonreturn heterospermic
2008/12/8
Despite the development of many new techniques, laboratory assays still do not predict male fertility accurately. To identify targets for laboratory assessment, we first need to determine which step...