搜索结果: 1-9 共查到“病理学 PCR”相关记录9条 . 查询时间(0.211 秒)
Detection and Prevalence of Polyoma Virus BK among Iranian Kidney Transplant Patients by a Novel Nested-PCR
BK virus Renal transplant recipients Prevalence Iran
2015/9/21
Background: BK virus is an increasingly recognized pathogen in transplant recipient patients associated with nephropathy and emerged as a cause of allograft failure linked to immunosuppressive regimen...
实时荧光反转录PCR检测呼吸道合胞病毒
实时荧光反转录PCR 呼吸道合胞病毒 检测方法
2012/11/22
目的 建立快速、准确、特异的实时荧光反转录PCR方法检测呼吸道合胞病毒(RSV).方法 根据RSV基因序列设计引物和探针并优化实时荧光反转录PCR反应体系，对686例临床标本进行检测，阳性结果测序验证.结果 本实验所建立实时荧光反转录PCR方法可准确、特异地检测RSV；686名呼吸道感染患儿中RSV感染达到16.5%(113/686).结论 本研究建立的RSV实时荧光反转录PCR方法快速、准...
Clinical Features of Cutaneous Leishmaniasis and Direct PCR-Based Identification of Parasite Species in A New Focus in Southeast of Iran
Cutaneous leishmaniasis Leishmania major Iran
2009/12/7
Background: To clinically characterize the cutaneous leishmaniasis and identify the causative parasite species in Mirjaveh, an important geographical region across the border of Iran-Pakistan at South...
目的：探讨用重组PCR技术对人单核细胞趋化蛋白-1（hμMCP-1）基因cDNA进行缺失突变，构建N末端缺失7个氨基酸的编码序列hμMCP-1突变体-hμMCP-1（7ND）cDNA，以期实现7ND基因治疗抑制MCP-1活性。 方法： 根据缺失前后的两段基因片段A和B分别设计两对引物即内引物与外引物，第一轮PCR反应通过每段各自的内外引物，分别获得加有互补末端的A+和B+DNA片段。然后进行第二轮...
联合应用实时定量RT-PCR和激光显微切割检测单个肝细胞RNA的表达（英文）
laser microdissction Real-time quantitative PCR RNA Hepatocytes
2009/5/8
联合应用实时定量RT-PCR和激光显微切割检测单个肝细胞RNA的表达（英文）。
目的：研究低浓度 N-甲基-N'-硝基-N-甲基亚硝基胍对人羊膜FL细胞部分基因表达的影响，以助于阐明MNNG引起细胞应答反应的基因及其调控机制。方法：用ABI公司的高通量实时荧光定量PCR方法，检测FL细胞在0.2 μmol/L MNNG处理后基因表达发生的改变。数据用ABI公司的SDS 2.1软件分析。结果：MNNG处理后，在检测的95个基因中，29个基因表达发生改变，其中14个基因下调2倍以...
高通量实时荧光定量RT-PCR方法验证FL细胞对苯并(a)芘7,8-二氢二醇9,10-环氧化物处理的差异表达基因
RT-PCR 低密度芯片 苯并(a)芘7,8-二氢二醇9,10-环氧化物 差异基因表达
2008/4/19
目的 验证由寡核苷酸微阵列检出的FL细胞对苯并(a)芘7,8-二氢二醇9,10-环氧化物(BPDE)处理的差异表达基因。方法 FL细胞分别经0.1％二甲亚砜（DMSO）和0.5 μmol·L-1 BPDE处理，以高通量实时荧光定量RT-PCR方法(TaqMan®低密度芯片)平行检测185个目标基因的相对表达水平。结果 BPDE处理组相对DMSO对照组，51个基因表达有差异，12个基因表...
IN VITRO SEMI-QUANTITATIVE DETERMINATAION OF HUMAN GAMMA-INTERFERON EXPRESSION BY RT-PCR
semi-quantitative IFN-γ PHA
2010/4/12
Secreted cytokines of Th1 (T-helper)/Th2 cells play an important role in the pathogenesis of many diseases. Th1 cells secrete predominantly IFN-γ and IL-2 which regulate cell-mediated immunity against...
The Detection of Dopamine Gene Receptors (DRD1-DRD5) Expression on Human Peripheral Blood Lymphocytes by Real Time PCR
Dopamine Receptors Gene Expression Lymphocytes Polymerase Chain Reaction
2010/2/8
There is interrelationship between the immune and nervous systems that is accomplished by the molecular mediators. Dopamine is one of the most important neurotransmitters. Five different dopamine rece...