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Polyamine profiles in rat testis, germ cells and Sertoli cells during testicular maturation
Polyamine profiles rat testis germ cells Sertoli cells
2009/2/23
Polyamine cellular concentrations (putrescine, spermidine and spermine) in the rat testis and testicular cell types were determined by fluorescence spectroscopy of their dansyl derivatives. A met...
Effect of germ cells on vectorial secretion of androgen binding protein and transferrin by immature rat Sertoli cells in vitro
germ cells vectorial secretion androgen binding protein transferrin immature rat Sertoli cells
2009/2/23
The influence of germ cells (greater than 85% pachytene spermatocytes) on vectorial secretion of androgen binding protein (ABP) and transferrin by immature rat Sertoli cells was investigated using t...
Germ cell degeneration in the contralateral testis of the guinea pig with unilateral torsion of the spermatic cord. Quantitative and ultrastructural studies
testis torsion quantitation germ cell guinea pig reproduction
2009/2/23
This study evaluated the long term effects of unilateral torsion of the spermatic cord on the contralateral testis of guinea pigs, employing both fine structural and quantitative studies. Young, adu...
Secretion of androgen binding protein by Sertoli cells is influenced by contact with germ cells
androgen binding protein Sertoli cells germ cells
2009/2/20
Sertoli cells were cultured alone or with germ cells to evaluate the effect of the association with germ cells on the secretory activity of Sertoli cells. Secretion of androgen-binding protein, whic...
Squash preparation studies of germ cells in human fetal testes
testis preleptotene germ cells chromosomes
2009/2/20
Testes from human fetuses 21 to 41 weeks of gestation were investigated using a modified squash technique. At all of the gestational ages examined, 90 to 95% of the germ cells were in interphase. Pr...
Pure Sertoli Cell Cultures：A New Model for the Study of Somatic-Germ Cell Interactions
cell cultures Sertoli cell germ cell
2009/2/19
A new technique involving a brief hypotonic treatment was developed for obtaining pure rat Sertoli cell cultures. This method for the selective removal of the germ cells present in Sertoli cell enri...
Reproductive aging in the Brown Norway rat is characterized by accelerated germ cell apoptosis and is not altered by luteinizing hormone replacement
Brown Norway rat accelerated germ cell apoptosis luteinizing hormone replacement
2009/2/18
Reproductive aging in the male Brown Norway (BN) rat is characterized by decreased Leydig cell steroidogenesis associated with seminiferous tubule dysfunction. This could be a result of a combination ...
Contrin, the human homologue of a germ-cell Y-box-binding protein：cloning, expression, and chromosomal localization
human homologue germ-cell Y-box-binding protein chromosomal localization
2009/2/18
Inactivation of germ-cell-specific molecules essential for the production of functional spermatozoa could lead to attractive new means for male contraception. The mouse protein MSY2 is the mammali...
Quantification of apoptotic testicular germ cells in normal and methoxyacetic acid-treated mice as determined by flow cytometry
Spermatogenesis TUNEL
2009/2/18
Several studies have reported the occurrence and significance of programmed cell death (apoptosis) of testicular germ cells in mammals. In those studies, apoptotic germ cells were identified by mo...
Evidence for cross-talk between Sertoli and germ cells using selected cathepsins as markers
cross-talk Sertoli germ cells
2009/2/18
To examine whether proteases are possibly involved in cellular migration and/or spermiation when developing germ cells translocate across the seminiferous epithelium during spermatogenesis, in sit...
Hormonal regulation of spermatogenesis in the hypophysectomized rat: quantitation of germ-cell population and effect of elimination of residual testosterone after long-term hypophysectomy
Testis germ cells androgen morphometry
2009/2/17
Spermatogenesis continues after long-term hypophysectomy (Hx), but massive cell degeneration prevents seminiferous tubules from attaining the full complement of cells. One objective of this study ...
Rat testicular germ cells and epididymal sperm contain active P450 aromatase
Estrogen testis epididymis
2009/2/17
Although testosterone is the principal sex steroid produced by the testis, estrogen is known to be produced by both Leydig and Sertoli cells during different developmental periods. Additionally, evide...
Spontaneous degeneration of testicular germ cells in congenitally athymic nude mice of four strains：a light microscopical observation
Spontaneous degeneration testicular germ cells
2009/2/17
Many hormonal studies have demonstrated that the presence of thymic factors affects testicular function. However, morphological studies on the testis in the absence of thymic factors are very limite...
Interactions of proteases and protease inhibitors in Sertoli-germ cell cocultures preceding the formation of specialized Sertoli-germ cell junctions in vitro
proteases and protease inhibitors Sertoli-germ cell specialized Sertoli-germ cell
2009/2/17
The biochemical mechanism(s) by which germ cells can form specialized junctions with Sertoli cells in the seminiferous epithelium at various stages of the spermatogenic cycle is unknown. This stud...
Is Leydig cell steroidogenic function affected by the germ cell content of the seminiferous tubules?
Leydig cell steroidogenesis
2009/2/17
The effect of testicular germ cell content on Leydig cell steroidogenic function in vivo in adult rats was examined. Three experimental paradigms were used to effect germ cell changes. First, a vita...