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Isolation and characterization of the plasma membrane of rat cauda epididymal spermatozoa
Isolation characterization plasma membrane rat cauda epididymal spermatozoa
2009/2/23
Cauda epididymal rat spermatozoa were isolated by flushing the excised epididymis and the plasma membrane was detached by a nitrogen cavitation treatment (500 psi, 10 minutes equilibration at 4 C)...
Identification and isolation of epididymal luminal proteins of the mouse
Identification isolation epididymal luminal proteins mouse
2009/2/23
Proteins of mouse cauda epididymal fluid were analyzed by polyacrylamide gel electrophoresis. Fluid expressed from the cauda epididymidis and samples obtained by micropuncture of the epididymal lume...
Characterization of boar sperm plasma membranes by two-dimensional PAGE and isolation of specific groups of polypeptides by anion exchange chromatography and lectin affinity chromatography
plasma membranes spermatozoa twodimensional
2009/2/19
High resolution, two-dimensional PAGE was used to characterize purified plasma membranes (PM) from boar spermatozoa. In addition to the abundance of polypeptides resolved, unusual features of PM dis...
Rat seminal-vesicle secretory protein SVS II binds DNA with a preference for the 5' regulatory region of secretory protein SVS IV gene：co-isolation with components of the nuclear matrix
caltrin nuclear scaffold nuclear structure semen tissue
2009/2/18
In rats, the ventral prostate and seminal vesicles produce distinct sets of proteins whose functions and tissue-specific regulation by androgens remain unclear. We have utilized the genes encoding t...
Single-step isolation of bovine seminal platelet-activating factor acetylhydrolase
Seminal plasma phospholipase A2 protein purification
2009/2/17
Bovine seminal platelet-activating factor acetylhydrolase was isolated to >90% purity in a single step using a butyl sepharose column. The procedure involves the elution of the activity by use of ...
Isolation of highly purified type A spermatogonia from prepubertal rat testis
Type A spermatogonia cell separation c-kit cell culture
2009/2/17
We have developed a new method that allows isolation of highly purified type A spermatogonia from prepubertal rats. The procedure is based on the maximal release of spermatogonia from the seminifero...
Isolation and characterization of mitochondria from turkey spermatozoa
Isolation characterization mitochondria turkey spermatozoa
2009/2/12
A procedure was developed to rapidly isolate functional, intact mitochondria from turkey spermatozoa. Semen was collected from turkeys, pooled, and centrifuged to remove spermiophages and other cell...
Immunomagnetic isolation and long-term culture of mouse type A spermatogonia
Germ cells c-kit Ep-CAM magnetic beads Sertoli
2008/12/29
In the mammalian testis, type A spermatogonia proliferate and differentiate into sperm under the tight control of both endocrine and paracrine factors. In order to study the complex process of sperm...
Recipient Preparation and Mixed Germ Cell Isolation for Spermatogonial Stem Cell Transplantation in Domestic Cats
Testis male radiation feline conservation
2008/12/23
The loss of genetic diversity poses a serious threat to the conservation of endangered species, including wild felids. We are attempting to develop spermatogonial stem cell transplantation in the cat ...
Spermatogonial Stem Cell Isolation, Storage, and Transplantation
Spermatogonial Stem Cell Isolation Storage Transplantation
2008/12/23
The past 30 years have been marked by unparalleled accomplishments in the medical treatment of malignancy. Prior to advances in chemotherapeutic and radiation treatment, many oncologic conditions had ...