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The Production of Antiserum against Myrobalan Latent Ringspot Virus for Detection of the Virus using ELISA
nepovirus MLRSV purification immunisation of rabbits
2015/3/2
Myrobalan latent ringspot virus (MLRSV) was purified from the extract of infected Chenopodium quinoa Willd.,
using n-octanol, differential centrifugation, and saccharose gradient centrifugation. Ele...
Quantification of Limonin Glucoside, Phytonutrient Component,in Citrus by Means of an Enzyme Immunoassay Using Anti-limonin Antiserum
bitterness EIA ELISA juice limonoid
2009/7/7
A solid phase enzyme immunoassay (EIA) for the quantitative determination of the phytonutrient component, limonin glucoside, in citrus juice was developed. Limonin glucoside and limonin were in aqueou...
Characterisation of a Polyclonal Antiserum Againts Ubiquitin and its use in the Investigation of Ubiquitin Conjugate Distribution in Normal and Stressed 3T3-LI Cells
Ubiquitin Heat Shock Stress
2009/7/2
Antiserum against ubiquitin was used to identify ubiquitin conjugates in 3T3- LI cells, and to investigate changes in the amount and distribution of these conjugates in cells subjected to stress. Stre...
Passive Immunization Approach to Reduce Body Fat in Pigs Using Fat-specific Polyclonal Antiserum
Pig Adipocytes Polyclonal Antiserum ELISA Carcass Percentage Body Composition
2016/4/26
Plasma membrane proteins from pig adipocytes, brain, heart, kidney, liver and spleen were isolated using a 32% sucrose gradient. An adult male sheep was immunized three times at 3-wk intervals with th...