搜索结果: 1-7 共查到“Ketosteroid Isomerase”相关记录7条 . 查询时间(0.055 秒)
Extreme Electric Fields Power Catalysis in the Active Site of Ketosteroid Isomerase
Electric Power Catalysis Active Site Ketosteroid Isomerase
2016/5/25
Enzymes use protein architecture to impose specific electrostatic fields onto their bound substrates, but the magnitude and catalytic effect of these electric fields have proven difficult to quantify ...
Quantitative Dissection of Hydrogen Bond-Mediated Proton Transfer in the Ketosteroid Isomerase Active Site
active site environment computational modeling enzyme catalysis protein electrostatics protein semisynthesis
2016/5/25
Hydrogen bond networks are key elements of protein structure and function but have been challenging to study within the complex protein environment. We have carried out in-depth interrogations of the ...
Site-Specific Measurement of Water Dynamics in the Substrate Pocket of Ketosteroid Isomerase Using Time-Resolved Vibrational Spectroscopy
IL-33 ST2 MWCNTs pulmonary fibrosis nanotoxicology
2016/5/25
Little is known about the reorganization capacity of water molecules at the active sites of enzymes and how this couples to the catalytic reaction. Here, we study the dynamics of water molecules at th...
Evaluation of the Energetics of the Concerted Acid-Base Mechanism in Enzymatic Catalysis:The Case of Ketosteroid Isomerase
Energetics Concerted Acid-Base Mechanism Enzymatic Catalysis Ketosteroid Isomerase
2016/5/25
Structures of enzymes invariably reveal the proximity of acidic and basic residues to reactive sites on the substrate, so it is natural and common to suggest that enzymes employ concerted mechanisms t...
Direct Measurement of the Protein Response to An Electrostatic Perturbation That Mimics the Catalytic Cycle in Ketosteroid Isomerase
hydroperoxide lipids GUV photosensitization methylene blue
2016/5/25
Understanding how electric fields and their fluctuations in the active site of enzymes affect efficient catalysis represents a critical objective of biochemical research. We have directly measured the...
Solvation Response Along the Reaction Coordinate in the Active Site of Ketosteroid Isomerase
Pseudomonas putida Coumarins Isomerases Spectrometry, Fluorescence Catalytic Domain Ketosteroids Biocatalysis Light Models, Molecular Equilenin
2016/5/25
A light-activated reaction analog has been developed to mimic the catalytic reaction cycle of Delta(5)-3-ketosteroid isomerase to probe the functionally relevant protein solvation response to the cata...
Proton Affinity of the Oxyanion Hole in the Active Site of Ketosteroid Isomerase
Pseudomonas putida Protons Steroid Isomerases Crystallography,X-Ray Nuclear Magnetic Resonance,Biomolecular Catalytic Domain Protein Conformation Substrate Specificity Ketosteroids Mutagenesis, Site-Directed
2016/5/25
The absorption spectra of a series of inhibitors bound at the active site of Delta(5)-3-ketosteroid isomerase from Pseudomonas putida were found to exhibit substantial variations in the contributions ...