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搜索结果: 1-13 共查到Prokaryotic相关记录13条 . 查询时间(0.049 秒)
安徽师范大学分子生物学英文课件Chapter6 Regulation of Prokaryotic Gene Expression。
My thesis comprises of two individual projects: 1) we have developed a database for operon prediction using high-throughput sequencing datasets for bacterial genomes. 2) Genomics and mechanistic insig...
Heterotrophic production and abundance of prokaryotes (i.e.,BacteriaandArchaea) in meso- (200–1000 m) and bathypelagic (.1000 m) zones were measured at 31 stations along meridional transects of the ce...
The highly adaptive nature of prokaryotic communities in the face of changing environmental conditions reflects in part their ability to share advantageous genetic information through horizontal gene ...
Bacterial biomarkers (D-amino acids and muramic acid) were investigated in surface sediments (0–1 cm) at 22 stations in the Peru margin. Concentrations were used to quantitatively estimate the relati...
The distribution of prokaryotic abundance (PA), respiratory activity (ETS), heterotrophic production (PHP), and suspended particulate (POM) and dissolved (DOM) organic matter was determined in the me...
The rapidly increasing number of potentially novel species, combined with the methodologically laborious polyphasic approach used in bacterial systematics, makes identification and, consequently, the ...
Isoprimeverose-producing oligoxyloglucan hydrolase (IPase; EC 3.2.1.120) is a unique β-glycosidase that cleaves xyloglucan oligosaccharides at the non-reducing end, producing isoprimeverose. Here, we ...
摘要 根据已报道的序列,把苦瓜(Momordica charantia) MAP30基因成功克隆到原核表达载体pET28a(+)中,并对PCR快速检测阳性克隆进行了研究。结果表明,直接用菌落、菌液、酚氯仿处理过的菌液,以及提取的质粒进行PCR都可以成功地筛选阳性菌落。其中,酚氯仿处理过的菌液PCR与质粒PCR的结果最接近,而且比质粒PCR简单,因此可作为方便可靠的阳性克隆筛选的新方法。Abstr...
摘要以酵母基因组DNA为模板,通过PCR方法分别扩增出在5′端带有6xHis标签的gcn5和rpd3基因,将其克隆到pBV220质粒中,分别构建成表达质粒pBVgcn5和pBVrpd3,转化大肠杆菌(Escherichia coli)并进行诱导表达。SDS-PAGE显示,含重组质粒的菌株经热诱导后分别过量表达出约50kDa的蛋白。利用6xHis亲和层析纯化了这两种重组酶。对组蛋白乙酰基转移酶GCN...
Myostatin is a member of the transforming growth factor-??(TGF-?? super-family. It acts as a negative regulator for skeletal muscle growth. Myostatin mutations are characterized by a visible, generali...
We measured prokaryotic production and respiration in the major water masses of the North Atlantic down to a depth of ~4,000 m by following the progression of the two branches of North Atlantic Deep W...
We measured relative bacterial and archaeal richness and its covariation with physical and other biological parameters during six cruises in the North Sea over prokaryotic productivity levels ranging ...

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