搜索结果: 1-15 共查到“SRY”相关记录29条 . 查询时间(0.053 秒)
中国农业科学院深圳农业基因组所阮珏团队发布性染色体组装新方法SRY(图)
阮珏团 植物基因 群体
2024/8/19
2024年4月16日,《GigaScience》在线发表了中国农业科学院深圳农业基因组研究所(岭南现代农业科学与技术广东省实验室深圳分中心)阮珏课题组发表的研究论文,题为“An effective strategy for assembling the sex-limited chromosome”。
日本研究人员发现决定性别的蛋白质Sry-T
日本 决定性别 蛋白质 Sry-T
2020/12/17
据日本《共同网》报道,日本大阪大学分子生物学教员宫胁慎吾研究团队10月1日在美国《科学》杂志网络版发表的研究成果称其发现了决定老鼠性别的蛋白质,该团队认为“有望弄清决定哺乳动物性别的机制,今后还将以人类为对象进行验证”。
Penile hypoplasia and rudimentary prepuce in a dog (78,XY;SRY-positive):a case report
dog disorder of sex development penile hypoplasia rudimentary prepuce SRY ZFX/ZFY
2016/6/3
This is the first report in the literature concerning penile hypoplasia and rudimentary prepuce in a 78,XY; SRY-positive male that received a successful surgical treatment. A six-month-old male mixed-...
SRY-negative XX sex reversal in an English Cocker Spaniel: a case report
English Cocker Spaniel intersexuality SRY-negative
2015/5/25
A SRY-negative XX male describes a female karyotype with testicular tissue resulting from abnormal
gonadal development. In such cases male gonad formation takes place in the absence of a Y chromosom...
SRY-negative XX sex reversal in an English Cocker Spaniel:a case report
English Cocker Spaniel intersexuality SRY-negative sex determination XX male
2015/4/30
A SRY-negative XX male describes a female karyotype with testicular tissue resulting from abnormal gonadal development. In such cases male gonad formation takes place in the absence of a Y chromosome ...
胃癌组织中 SRY-Box2 基因的表达与其甲基化的关系
SRY-Box2 基因 甲基化 胃癌
2014/4/28
检测干细胞转录因子 SRY-Box2 基因在胃癌MKN74、MKN45细胞株及人胃癌组织标本中的mRNA表达水平与甲基化情况,分析其与胃癌发生及临床病理特征之间的相关性。 方法: 选取河北医科大学第四医院胸外科2007至2012年收治的胃癌患者86例,取癌组织原发灶及其癌旁组织;分别用5-氮杂-2′-脱氧胞苷(5-aza-2′-deoxycytidine,5-Aza-Dc)与曲古抑菌素A(tric...
Detection of the SRY Transcript and Protein in Bovine Ejaculated Spermatozoa
SRY Gene Bovine Spermatozoa In situ Hybridization Bovine
2016/5/9
The sex-determining region on the Y (SRY) gene is important in mammalian sex determination and differentiation. We report a study of the abundance of SRY gene products in bovine ejaculate. RT-PCR expe...
性别决定基因Sry原核表达载体的构建和蛋白检测
性别决定 Sry 原核表达载体 蛋白表达
2009/8/3
动物的性别在畜牧业是一项很重要的经济性状,而哺乳动物的性别决定受睾丸决定基因Sry的启动,获得SRY蛋白并探索它与其他相关因子之间的相互关系对于深入了解该因子的作用机理和寻求高效经济的性别控制方法尤为重要。本研究以小鼠为实验材料,通过PCR、质粒重组、酶切和测序等方法构建小鼠Sry的原核表达载体,使用IPTG诱导蛋白表达、SDS-PAGE电泳和western blotting等方法检测重组表达载体...
利用SRY基因鉴定山羊胎儿成纤维细胞的性别
SRY基因 山羊胎儿成纤维细胞 性别鉴定
2009/7/14
根据GenBank中已发表的山羊SRY基因和β乳球蛋白基因序列设计2对PCR引物,对来自3个1月龄山羊胎儿成纤维细胞的基因组DNA进行PCR扩增,分别以公羊、母羊的基因组DNA为阳性和阴性对照,同时对PCR产物进行序列测定和同源性分析。结果表明,阳性对照和GFF1细胞系有2条扩增带,阴性对照、GFF2和GFF3细胞系只有1条扩增带;序列测定结果表明,PCR扩增产物为SRY基因,利用该方法准确...
Detection of the SRY Gene in a 46,XY Phenotypic Female by the PCR-SSCP Method
PCR-SSCP Sex reversal SRY 46 XY Female
2009/6/30
The short arm of the human Y chromosome determines maleness, initiating the development of the testes. Mutations and deletions in this region affect gonadal differentiation, causing primary sex revers...
Amplification of Porcine SRY Gene for Sex Determination
SRY Gene Sex Determination Sperm Sorting Duplex PCR
2016/4/29
The separation of X and Y chromosome-bearing sperm is of use in many aspects of livestock maintenance. In this study, we sought to determine the difference in DNA content between X- and Y-bearing sper...
Discrimination of Korean Cattle (Hanwoo) Using DNA Markers Derived from SNPs in Bovine Mitochondrial and SRY Genes
Hanwoo Bos taurus Bos indicusx Bos indicus PCR-RFLP SRY ND4 ND5
2016/4/27
In order to distinguish Korean cattle (Hanwoo) beef from the imported beef from Australia in Korean markets, DNA markers based on PCR-RFLP from mitochondrial genes and SRY gene were applied. A total o...
PCR扩增泥鳅和大鳞副泥鳅SRY盒基因
SRY盒 基因 泥鳅 大鳞副泥鳅
2008/3/13
以特异扩增人SRY基因保守区的一对引物,研究了泥鳅和大鳞副泥鳅基因组中SRY盒基因的扩增。结果表明,该引物可以在泥鳅中扩增出四条带,其长度分别为200, 550、940和1000bp。在大鳞副泥鳞中扩增出三条带,大小为200,550和900bp。经Southern杂交显示出二者的阳性带为200和550bp
p。阳性带在雌雄个体间和两个物种间无差异。
摘要应用显微切割技术获得赤麂1号、Y1、Y2染色体,通过DOP-PCR增加模板DNA拷贝数,然后用人的性别决定基因(Sex-determining Region of the Chromosome Y,SRY)中HMG框内设计1对引物,对DOP-PCR产物进行扩增。在雄性赤麂Y2染色体DOP-PCR产物中扩增出与人SRY基因同源的Sry基因片段,克隆、测序,首次在分子水平上证明赤麂Y2染色体是真正...