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Cloning and Expression of Yak Active Chymosin in Pichia pastoris
Active Chymosin Yak Expression Pichia pastoris
2016/11/10
Rennet, a complex of enzymes found in the stomachs of ruminants, is an important component for cheese production. In our study, we described that yak chymosin gene recombinant Pichia pastoris strain c...
Effects of Starvation on Lipid Metabolism and Gluconeogenesis in Yak
Starvation Maiwa Yak Fat Catabolism Ketone Bodies Gluconeogenesis
2016/11/10
This research was conducted to investigate the physiological consequences of undernourished yak. Twelve Maiwa yak (110.3±5.85 kg) were randomly divided into two groups (baseline and starvation group)....
Screening of Probiotic Activities of Lactobacilli Strains Isolated from Traditional Tibetan Qula, A Raw Yak Milk Cheese
Probiotic Activities Screening Lactobacilli Qula Cheese
2016/11/10
In this study, 69 lactobacilli isolated from Tibetan Qula, a raw yak milk cheese, were screened for their potential use as probiotics. The isolates were tested in terms of: Their ability to survive at...
Comparative Proteomic Analysis of Yak Follicular Fluid during Estrus
Yak Follicular Fluid Two-dimensional Gel Electrophoresis Mass Spectrometry Western Blot
2016/11/10
The breeding of yaks is highly seasonal, there are many crucial proteins involved in the reproduction control program, especially in follicular development. In order to isolate differential proteins b...
Melatonin receptor (MTNR1A and MTNR2B) expression during the breeding season in the yak (Bos grunniens)
domestic yak immunofluorescence MLTR
2015/7/3
Melatonin plays key roles in a wide range of mammalian body functions, which are mediated by the melatonin-specific cell surface receptor (MTNR1A and MTNR1B). To better understand the role of MTNR in ...
Expression profiles of myostatin and calpastatin genes and analysis of shear force and intramuscular fat content of yak longissimus muscle
Bos grunniens meat trait muscle growth hypoxia intramuscular fat
2015/6/9
The main objective of this study was to reveal the expression profiles of two negative regulators, myostatin (MSTN) and calpastatin (CAST)genes, of skeletal muscle growth in highland yaks (Bos grunnie...
Molecular Cloning and Characterization of a New C-type Lysozyme Gene from Yak Mammary Tissue
Yak Mammary Tissue Milk Lysozyme Recombinant Protein
2016/5/19
Milk lysozyme is the ubiquitous enzyme in milk of mammals. In this study, the cDNA sequence of a new chicken-type (c-type) milk lysozyme gene (YML), was cloned from yak mammary gland tissue. A 444 bp ...
Molecular Characterization and Tissue Distribution of Estrogen Receptor Genes in Domestic Yak
Yak Estrogen Receptor Gene Gene Expression Tissue Distribution
2016/5/17
Estrogen and its receptors are essential hormones for normal reproductive function in males and females during developmental stage. To better understand the effect of estrogen receptor (ER) gene in ya...
Comparison of Nitrogen Metabolism in Yak (Bos grunniens) and Indigenous Cattle (Bos taurus) on the Qinghai-Tibetan Plateau
Yak Grazing Intake Nitrogen Balance Plasma Urea Nitrogen Purine Derivative
2016/5/6
The objective of the present study was to examine whether yaks possess any adaptive mechanisms of nitrogen (N) metabolism to survive in the harsh foraging environment of the Qinghai-Tibetan Plateau. A...
Evaluation of Chemical Composition and In vitro Digestibility of Appennine Pasture Plants Using Yak (Bos grunniens) Rumen Fluid or Faecal Extract as Inoculum Source
Yak Pasture Quality Faecal Extract In vitro Digestibility
2016/5/5
Pastures of the Apennines of Central Italy contribute to feed resources of high altitude (above 1,300 m sea level) grazing systems. The objective of this study was to evaluate the effectiveness of fae...
Indigenous yak and yak-cattle crossbreed management in high altitude areas of northern Nepal: A case study from Rasuwa district
Indigenous knowledge yak and hybrids management system
2010/9/13
This paper summarizes and documents the indigenous yak and chauri managements in high altitude mountainous areas of Nepal through on-the-spot surveys on the farmers from three villages of case study s...
Milking performance of China yak(Bos grunniens):A preliminary report
China yak breeds milk performance
2009/3/3
Yak milk plays a very important role in Tibetan’s daily life. Milk and milk products are the major dietary ingredients as well as family incomes of Tibetan herders in China. Yak milk yield and compo...
Cloning and Sequence Analysis of Glyceraldehyde-3-Phosphate Dehydrogenase Gene in Yak
Yak Glyceraldehyde-3-Phosphate Dehydrogenase gene Cloning Housekeeping Gene
2016/4/28
In order to study the biological function of gapdh gene in yak, and prove whether the gapdh gene was a useful intra-reference gene that can be given an important role in molecular biology research of ...
摘要为进一步筛选、克隆和表达牦牛相关基因构建了牦牛肝脏的cDNA文库,从牦牛肝脏中提取、分离mRNA,逆转录合成cDNA后,以pSPORT1质粒为载体构建cDNA文库。经鉴定,牦牛肝脏cDNA 文库的库容量为1×106转化子/μg cDNA,以菌株平板法测定,重组率为93%。所构建的cDNA 文库容量符合建库要求,能够用于目的基因筛选。
Abstract:Yak is a very useful...
期刊信息
篇名
Genetic diversity and divergence in Chinese yak(Bos grunniens)populations inferred from blood protein electrophoresis
语种
英文
撰写或编译
作者
Tu Z C,Zhang Y P,Qiu H
第一作者单位
刊物名称
Biochem Genet
页面
1997,34...