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搜索结果: 1-13 共查到genomic DNA相关记录13条 . 查询时间(0.108 秒)
The process of generating RNA message from a gene is termed "transcription," and is performed by aptly named "transcription factors." These transcription factors can function singly or in conjunction ...
The process of generating RNA message from a gene is termed "transcription," and is performed by aptly named "transcription factors." These transcription factors can function singly or in conjunction ...
The aim of the present study was to develop a very fast and simple genomic DNA isolation method for Ralstonia solanacearum which infest potato tubers. One hundred potato tubers were collected and ten ...
Epitope tagging has been widely used for studies of protein structure and function (8). The epitope tagging process generally begins with a cDNA encoding the protein of interest. A major weakness...
In the course of cloning of bovine cDNA for proline-rich protein (PRP) P-B from bovine tooth germ cDNA, we found that one clone with 662 bp contained a 5'-terminal 393 bp (1-393 bp) sequence essenti...
A fast, simple, and reliable mini-prep method for the extraction of DNA from Gossypium species and cultivars has been developed. This small-scale method is cetyltrimethylammonium bromide (cTAB)-based,...
Among the transcribed regions of a gene, introns are the most polymorphic; therefore, they are ideally suited for developing molecular markers. The identification of intron regions, however, is not a ...
摘要 灰树花是一种珍贵的药用真菌,因为多糖含量较高,较难获得高质量的总DNA,本文提出了一种制备高质量灰树花总DNA及构建灰树花基因组文库的方法。该方法制备的灰树花总DNA,经Sau3AI酶切后,用于构建基因组文库,可得到2×105个转化子/50mg,平均插入片段为14kb。本研究为下一步克隆灰树花中的基因以及进行其他分子生物学研究奠定了基础。Abstract: Grifola frondos...
根据多年生植物组织富含多酚、多糖的具体特性,对现有的DNA提取方法进行了改进。通过增加提取缓冲液中b-巯基乙醇用量,简化氯仿/异戊醇抽提液步骤,改用经-20℃预冷异丙醇沉淀DNA等,对CTAB法加以改进。改进后方法具有以下优点:(1)获得的DNA质量良好,提取过程无明显的DNA降解,基本上排除了多酚物质的干扰;(2)用获得的DNA进行Southern杂交,可得到理想的杂交信号,可满足相关的分子研究...
专著信息 书名 Diversity SSH-array for profiling genomic DNA polymorphisms. 语种 英文 撰写或编译 作者 Tong Xiang Li,JinKe Wang,ZuHong Lu. 第一作者单位 出版社 Genomics 出版地 出版日期 2004年 月 日 标准书号 介质类型 页数 字数 开本 相关项目 名贵中药(石斛)gDNA种质鉴定芯...
期刊信息 篇名 Identification f Wheat-Haynaldia villosa amphiploid ,addition ,substitution and translocation lines by in situ hybridization Using biotin labeled genomic DNA as a probe 语种 英文 撰写或编译 撰写 作者 Chen ...
期刊信息 篇名 An efficient protocol for genomic DNA extraction from citrus species 语种 英文 撰写或编译 作者 Cheng YJ,Guo WW,Yi HL,Pang XM,Deng XX 第一作者单位 刊物名称 Plant Molecular Biology Reporter 页面 21(2): 177-178 (2003 出...
Background: Methylation of genomic DNA is dependent on an adequate supply of folate coenzymes. Previous data support the hypothesis that abnormal DNA methylation plays an integral role in carcinoge...

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