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中国科学院动物研究所重点研究领域
中国科学院动物研究所 重点研究领域 干细胞 生殖生物学 膜生物学 生物灾害 物种濒危保护 物种形成
2022/5/26
中国科学院动物研究所重点研究领域。1. 干细胞和生殖生物学研究领域：面向生命科学前沿和国家在人口健康领域的重大需求，依托“干细胞与再生医学创新研究院”和“干细胞与生殖生物学国家重点实验室”，建设国际一流的科研设施、平台和装置，并积极建议和参与国家实验室建设，提出引领性的国际重大科学计划和工程；围绕影响国民健康的重大疾病，重点解决生命发生、发育和修复的重大科学问题，研发干细胞与再生医学新技术、开发干...
DNA-Based Patterning of Tethered Membrane Patches
DNA Lipids Lipid Bilayers Molecular Structure Particle Size Surface Properties
2016/5/25
Solid-supported lipid bilayers are useful model systems for mimicking cellular membranes; however, the interaction of the bilayer with the surface can disrupt the function of integral membrane protein...
Individual Vesicle Fusion Events Mediated by Lipid-anchored DNA
Cholesterol DNA Lipid Bilayers Phosphatidylcholines Phosphatidylethanolamines Membrane Fusion Unilamellar Liposomes
2016/5/25
Membrane fusion consists of a complex rearrangement of lipids and proteins that results in the merger of two lipid bilayers. We have developed a model system that employs synthetic DNA-lipid conjugate...
Formation and Analysis of Topographical Domains Between Lipid Membranes Tethered by DNA Hybrids of Different Lengths
Cell Membrane DNA Membrane Lipids Lipid Bilayers 1,2-Dipalmitoylphosphatidylcholine Microscopy, Fluorescence Base Sequence Molecular Sequence Data Models,Chemical Unilamellar Liposomes
2016/5/25
We recently described a strategy to prepare DNA-tethered lipid membranes either to fixed DNA on a surface or to DNA displayed on a supported bilayer [Boxer et al., J. Struct. Biol., 2009, 168, 190; Bo...
Stability of DNA-Tethered Lipid Membranes with Mobile Tethers
Metallic glasses Rapid-solidification Microstructure Mechanical properties
2016/5/25
We recently introduced two approaches for tethering planar lipid bilayers as membrane patches to either a supported lipid bilayer or DNA-functionalized surface using DNA hybridization (Chung, M.; Lowe...
Covalent Attachment of Lipid Vesicles to a Fluid-Supported Bilayer Allows Observation of DNA-Mediated Vesicle Interactions
Caspian and Black Sea waters reverse-osmotic desalination Na-zeolite softening of the permeate
2016/5/25
Specific membrane interactions such as lipid vesicle docking and fusion can be mediated by synthetic DNA-lipid conjugates as a model for the protein-driven processes that are ubiquitous in biological ...
DNA-tethered Membranes Formed by Giant Vesicle Rupture
Tethered lipid membrane Surface immobilized DNA Giant unilamellar vesicles
2016/5/25
We have developed a strategy for preparing tethered lipid bilayer membrane patches on solid surfaces by DNA hybridization. In this way, the tethered membrane patch is held at a controllable distance f...
A Membrane Interferometer
bilayer lipid membrane black lipid membrane fluorescence interference contrast
2016/5/25
Freestanding phospholipid bilayers have been assembled spanning shallow, micrometer-sized wells etched into a Si wafer substrate so that the bilayers are near (within hundreds of nanometers) but not i...
Advances in Imaging Secondary Ion Mass Spectrometry for Biological Samples
membrane organization chemical composition imaging NanoSIMS ToF-SIMS dynamic SIMS
2016/5/25
Imaging mass spectrometry combines the power of mass spectrometry to identify complex molecules based on mass with sample imaging. Recent advances in secondary ion mass spectrometry have improved sens...
Kinetics of DNA-mediated Docking Reactions Between Vesicles Tethered To Supported Lipid Bilayers
DNA electron transfer oxidative stress redox regulation
2016/5/25
Membrane-membrane recognition and binding are crucial in many biological processes. We report an approach to studying the dynamics of such reactions by using DNA-tethered vesicles as a general scaffol...
Phase Separation of Lipid Membranes Analyzed with High-Resolution Secondary Ion Mass Spectrometry
Cell Membrane Membrane Microdomains Membrane Lipids Lipid Bilayers Phosphatidylcholines Membrane Proteins Spectrometry, Mass, Secondary Ion Nanotechnology
2016/5/24
Lateral variations in membrane composition are postulated to play a central role in many cellular events, but it has been difficult to probe membrane composition and organization on length scales of t...
Quantitative Analysis of Supported Membrane Composition Using the NanoSIMS
lipid bilayer SIMS NanoSIMS mixture gradient compositional analysis
2016/5/24
We have improved methods reported earlier [1] for sample preparation, imaging and quantifying components in supported lipid bilayers using high-resolution secondary ion mass spectrometry performed wit...
Probing the Structure of Supported Membranes and Tethered Oligonucleotides by Fluorescence Interference Contrast Microscopy
Water Oligonucleotides Microscopy, Fluorescence Microscopy, Interference Membranes, Artificial Sensitivity and Specificity Surface Properties Models, Chemical Silicon Dioxide
2016/5/24
Fluorescence interference contrast microscopy (FLIC) is a powerful method to structurally characterize fluorescent objects with nanometer-scale resolution in the z direction. Here we use FLIC to chara...
General Method for Modification of Liposomes for Encoded Assembly on Supported Bilayers
Liposomes Encoded Assembly Bilayers
2016/5/24
We recently reported a novel system shown schematically in Figure 1A in which intact lipid vesicles were assembled on a fluidsupported bilayer using oligonucleotide tethers.1 Functionalized oligonucle...
Patterned Supported Lipid Bilayers and Monolayers on Poly(dimethylsiloxane)
cyclohexanone monooxygenase sulphide oxidation biocatalysis engineered whole cells
2016/5/24
A simple and practical method for patterning supported lipid bilayers on poly(dimethylsiloxane) is presented. By using electron microscopy grids to laterally control the extent of plasma oxidation, th...