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In Vitro Fabrication and Biocompatibility Assay of a Biomimetic Osteoblastic Niche
Osteoblasts Biomimetic niche Hematopoietic stem cell expansion Dynamic environment Cell function maintain
2023/3/1
A novel HAp-CS/Gel biomimetic osteoblastic niche was fabricated by freeze-drying, and its mechanical strength and biocompatibility were characterized. HAp-CS/Gel scaffolds in various ratios of 100:5, ...
Development of decellularized meniscus extracellular matrix and gelatin/chitosan scaffolds for meniscus tissue engineering
Meniscus extracellular matrix compressive elastic modulus cytotoxicity
2023/3/1
Meniscus tissue engineering has provided a great potential treatment for meniscal injuries. However, few scaffolds in meniscus tissue engineering have matched the mechanical properties of native menis...
Effects of concentration variation on the physical properties of alginate-based substrates and cell behavior in culture
Alginate Cell encapsulation Gelatin
2023/3/1
Nowadays alginate capsules exhibit good biocompatibility and high permeability for nutrients and metabolic wastes making them appealing biomaterial for therapeutic cell encapsulation. Further study of...
3D Bio-Printing of CS/Gel/HA/Gr Hybrid Osteochondral Scaffolds
3D printing bio-ink graphene chitosan gelatin hyaluronic acid cartilage repair
2023/3/1
Cartilage is an important tissue contributing to the structure and function of support and protection in the human body. There are many challenges for tissue cartilage repair. However, 3D bio-printing...
Simultaneous expansion and harvest of hematopoietic stem cells and mesenchymal stem cells derived from umbilical cord blood
生物反应器 UCB MSCs RWV GCSC
2023/3/1
The simultaneous expansion and harvest of hematopoietic stem cells and mesenchymal stem cells derived from umbilical cord blood were carried out using bioreactors. The co-culture of umbilical cord blo...
为评价60Co-γ射线和电子束辐照灭菌对葛根提取物品质的影响效应及其差异性,采用不同剂量的60Co-γ射线(0、2.8、5.7、8.3、10.6 kGy)和电子束(0、2.4、5.1、7.7、10.3 kGy)辐照处理葛根提取物,研究辐照对其微生物总数、抗氧化活性及指纹图谱的影响。结果表明,60Co-γ射线和电子束辐照均能有效降低葛根提取物中需氧菌总数、霉菌和酵母菌总数,随着吸收剂量增大,抑制作用...
黄花蒿提取物的生物活性作用研究进展
黄花蒿 提取物 生物活性 研究进展
2020/7/15
黄花蒿是传统中草药之一,具有清热、解暑和治疗疟疾等功效。近些年,黄花蒿及其提取物因其显著的抑菌、促生长、调节免疫和抗氧化功能等作用受到了广泛关注。本文主要对黄花蒿提取物的生物活性作用进行综述,旨在为更好地将黄花蒿提取物应用在畜牧业中提供理论依据。
为建立中华鳖背甲中胶原蛋白酶解物(CH)的制备条件,并明确其体外抗氧化活性,本研究采用单因素试验探讨鳖甲胶原蛋白酶解物的分离条件。结果表明,其最佳提取工艺参数:胃蛋白酶添加量 4 000 U·g-1、pH值2.5、酶解温度35℃。通过透析纯化后获得体外抗氧化活性最好的组分为分子量<50 kDa的样品。该样品经Sephadex G-75凝胶色谱分离纯化得到2个组分,其中GP-2组分对O2-·、OH·...
为考察祁白术多酚(polyphenols from Atractylodes macrocephala Koidz grown in Qimen, AMP)的可利用性,采用响应面分析法优化酶法提取工艺,并研究AMP的抗氧化、抑制黑色素合成活性。结果表明,在料液比1:30 g·mL-1、酶解时间20 min的条件下,当纤维素酶添加量为1.35%、酶解温度为44℃、pH值为4.7、搅拌转速为670 r...
冷榨芝麻饼粕木脂素提取及其抗氧化研究
冷榨芝麻饼粕 木脂素 抗氧化 食用油
2020/3/16
为有效提高芝麻冷榨副产物的利用价值,以芝麻冷榨制油后的饼粕为原料,乙醇为溶剂,采用超声波辅助法提取芝麻饼粕木脂素,选取乙醇浓度、料液比、提取时间、提取功率4个考察因素,在单因素试验的基础上应用响应面法对芝麻饼粕木脂素提取工艺条件进行优化,并应用Schaal烘箱法,以过氧化值(PV)、茴香胺值(AV)及全氧化值(TV)为指标,对添加木脂素的食用油进行抗氧化活性评价。结果表明,优化后芝麻饼粕木脂素的提...
不同产地海带脂肪酸组成的比较分析
海带 总脂 脂肪酸 产地
2020/3/17
为比较分析不同产地海带的脂肪酸组成,本研究采用溶剂法对海带总脂提取条件进行优化,并通过气相色谱-质谱技术对福州、宁波和青岛3个产地海带的总脂脂肪酸组成进行比较分析。结果表明,二氯甲烷-甲醇法提取海带总脂的效率较高,是海带总脂提取的理想方法;福州、宁波和青岛海带的总脂含量依次为0.75%、0.69%和0.86%,且无显著差异。从海带总脂中共鉴定出30种脂肪酸,以 C16:0、C18:1n-9、C20...