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所谓眼见为实，在原位研究细胞内分子机器与超微结构，解析其复杂精密的组装结构与生理功能之间的关系，是生命科学的前沿热点。冷冻电子断层扫描成像（cryo-ET）是目前主要的原位研究技术，但受电子束穿透能力限制，需要将细胞和组织样品减薄成200纳米左右的薄片后成像。
植物需依靠叶绿素来吸收太阳辐射中可见光波段（400-700纳米）的光谱进行光合作用，该波段的太阳光通常被称为光合有效辐射（photosynthetically active radiation,PAR）。PAR是植物生命活动、有机物质合成和产量形成的能量来源，控制着陆地生物有效光合作用的速度，直接影响到植物的生长、发育、产量与产量质量。作为影响植物生长的主要生态因子，PAR还是陆地生态系统中碳循环...
Rapid Isolation of Bacterial Photosynthetic Reaction Centers with an Engineered Poly-Histidine Tag
photosynthetic reaction center poly-histidine tag isolation method (bacterium)
2016/5/23
A very rapid method for isolating bacterial photosynthetic reaction centers (RCs) of high purity and yield is described. A poly-histidine tag is engineered at the C-terminus of the RC M-subunit, allow...
Photosynthetic Reaction Center Mutagenesis via Chimeric Rescue of a Non-functional Rhodobacter capsulatus puf Operon with Sequences from Rhodobacter sphaeroides
photosynthesis recombination bacteria energy transfer antenna
2016/5/23
Photosynthetically active chimeric reaction centers which utilize genetic information from both Rhodobacter capsulatus and Rb. sphaeroides puf operons were isolated using a novel method termed chimeri...
The Effect of Very High Magnetic Fields on the Reaction Dynamics in Bacterial Reaction Centers:Implications for the Reaction Mechanism
Reactioncenter Photosynthesis Bacterialphotosynthesis Delayedfluorescence Magnetic fieldeffect (Rb. sphaeroides)
2016/5/23
Magnetic field effects have proven to be a powerful tool for elucidating the mechanism of the initial charge separation and recombination steps in bacterial photosynthesis. Since the first data were o...
The Effect of Very High Magnetic Fields on the Delayed Fluorescence from Photosynthetic Reaction Centers
Reactioncenter Photosynthesis Bacterialphotosynthesis Delayedfluorescence Magneticfieldeffect (Rb. sphaeroides)
2016/5/23
Delayed fluorescence is observed from quinone-depleted Rhodobacter sphaeroides reaction centers due to re-formation of the excited state of the special pair electron donor from the initially formed ra...
Stark Effect Spectroscopy of Rhodobacter Sphaeroides and Rhodopseudomonas Viridis Reaction Centers
Biophysics Photosynthesis Electron Transfer Electroabsorption Electrochromism
2016/5/20
The nature of the initially excited state of the primary electron donor or special pair has been investigated by Stark effect spectroscopy for reaction centers from the photosynthetic bacteria Rhodops...
Magnetic Field Dependence of Radical Pair Decay Kinetics and Molecular Triplet Quantum Yield in Quinone-Depleted Reaction Centers
Photosynthesis Reaction center Radical pair decay Magnetic field Picosecond spectroscopy
2016/5/20
Radical-pair decay kinetics and molecular triplet quantum yields at various magnetic fields are reported for quinone-depleted reaction centers from the photosynthetic bacterium Rhodopseudomonassphaero...
Photosynthesis involves a very large number of photochemical and chemical transformations. This review focuses on the extraordinarily rapid transport of excitation energy in the antenna pigmentprotein...
Picosecond Spectroscopic Study of Chlorophyll-Based Models for the Primary Photochemistry of Photosynthesis
Picosecond Spectroscopic Study Chlorophyll-Based Models Primary Photochemistry Photosynthesis
2016/5/20
A series of covalently linked dimers and trimers of chlorophyllide derivatives was investigated by time-resolved absorption and fluorescence spectroscopy (3-lo4 ps). For these compounds, the freeenerg...
Plasmon Excitation on a Metal Grating and its Phase Detection Based Biosensor Application
biosensors diffraction gratings excited states optical sensors plasmons refractive index 10< sup> -7< /sup>
2016/12/2
A grating-based biosensor via phase detection is stated. The resolution will be 10-7 RIU in the state-of-the-art today. The workspace can be shifted in a large range while the sensor resolution can re...
浅议激光的生物学效应及生物物理机理探讨
激光 激光生物效应 激光与生物分子
2011/11/24
本文主要简介了激光与生物组织相互作用所产生的生物效应，概述激光与生物分子相互作用机理研究现状。为提高和发展激光技术在此领域的应用，有必要对激光的生物学效应及生物物理机理进行研究。
病原菌Pst DC3000侵染拟南芥导致自噬和光合作用的抑制
丁香假单胞菌番茄致病变种 拟南芥 光合功能 细胞自噬 活性氧
2011/7/29
在病原菌侵染下，植物体是如何通过超敏反应限制病原菌扩增的，到目前为止还不清楚。最近的研究显示自噬起了必要的作用，可见，研究自噬和超敏反应的机制非常重要。本研究通过观察在非寄主病原菌突变体丁香假单胞菌番茄致病变种(Pseudomonas syringae pv. tomato DC3000，Pst DC3000)的侵染下，拟南芥光合功能的变化及自噬现象出现的情况，以为进一步研究植物抗病机理提供实验基...
针对CO2阶跃变化下茄子光合的振荡动态现象，采用CO2阶跃起点（Ca1）和阶点（Ca2）处理，以及不同光照水平（PAR）下的CO2阶跃处理，结合光合系统反馈控制动态生化模型中传递函数的振荡单元，分析了茄子动态光合的振荡特征参数。通过稳态光合参数，将茄子光合速率（Pn）转化为羧化速率（Vc）的动态进程，发现在较低的CO2阶跃起点（Ca1≤180 μmol/L）下，光合系统的时间常数T较大，光合系统的...
Collapse suppression and stabilization of dipole solitons in two-dimensional media with anisotropic semi-local nonlinearity
Collapse suppression dipole solitons anisotropic semi-local nonlinearity
2010/4/6
We consider the impact of anisotropic nonlocality on the arrest of the collapse and stabilization of dipole-mode (DM) solitons in two-dimensional (2D) models of optical media with the diffusive nonlin...