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Characterization of the human GYS2 gene and its product using bioinformatic tools
Glycogen synthase genomics evolution promoter expression
2009/7/6
Aim: The GYS2 gene, which encodes for glycogen synthase 2 (liver) (GS), is an enzyme responsible for the synthesis of 1,4-linked glucose chains in glycogen. The present study aimed to investigate th...
Investigation of the Inhibition Effects of Some Antibiotics on Human Erythrocyte Carbonic Anhydrase Isozymes
Carbonic anhydrase isozymes antibiotics inhibition
2009/7/2
In this study, the in vitro effects of some antibiotics on carbonic anhydrase (CA) isozymes have been investigated. Human erythrocyte CA-I and CA-II isozymes were separately purified by affinity chrom...
Transfection of Human Kidney Epithelial 293 Cells With Bacterial Cytosine-5-Methyltransferase M.Msp1 and The SV40 Nuclear Localisation Sequence of VP1
Kidney epithelial 293 cells C-5 MTase M.Msp1 NLS-VP1 cell transfection
2009/6/30
The aim of this study was to con-struct a plasmid that would direct the expres-sion of a bacterial DNA methyltransferase in cultured mammalian cells. It was carried out with a PCR-based strategy for i...
The Effects of Sodium Selenite on the Antioxidative Defence Mechanism of Human Hepatoma G2 Cell
Hepatoma G2 Sodium selenite Glutathione peroxidase Catalase Superoxide dismutase
2009/6/30
Human cells that live in biological harmony can become cancerous. As they do so, they engage in anaerobic respiration, undergo rapid cell growth and division according to nonmitotic rules, and importa...
Investigation of Various Terminal Carbohydrate Structures in Apolipoproteins of Human Serum Chylomicrons and VLDL
Lipoprotein apolipoprotein glycoprotein carbohydrate sequence
2009/6/30
The roles of terminal carbohydrate moieties of serum glycoproteins in their recognition and uptake by receptors and in their rapid removal from the circulation are well established. However, little is...
Analysis of ras Gene Mutation in Human Oral Tumours by Polymerase Chain Reaction and Direct Sequencing
Oral tumour PCR Direct sequencing Tobacco-specific nitrosamines
2009/6/29
Genetic alterations in proto-oncogenes or tumour suppressor genes are believed to be one of the key events in the multistage process of carcinogenesis. Activating point mutations occurring in either o...
Production of Monoclonal Antibody Specific for Human Hemoglobin
Institue for Genetic Engineering and Biotechnology,TÜ Bİ TAK Marmara Research Center,Gebze,Kocaeli Department of Medical Biology,Faculty of Medicine,Marmara University,İ stanbul-TURKEY
2009/6/29
Hemoglobin is not normally present in sputum, urine or feces except in minute amounts. Demonstration of the presence of hemoglobin in these materials is an important diagnostic clue that usually impli...
Purification and Characterization of Carbonic Anhydrase from Human Erythrocyte Plasma Membrane
Carbonic anhydrase Plasma membrane sulfanilamide KSCN and NaN3
2009/6/29
Carbonic anhydrase is the basic enzyme in inhalation function. Untill now no research had been done to determine whether CA is in the human erythrocyte plasma membrane or not. Carbonic anhydrase (CA) ...
Production and Purification of Monoclonal Antibodies to Human Interleukin-2 and Their Use for Immunoaffinity Purification of Recombinant Human Interleukin-2
Monoclonal antibody human interleukin-2 immunoaffinity purification
2009/6/26
Monoclonal antibodies (mAbs) are a powerful immunochemical tool. Today, the availability of mAbs with desired specificity enables ligand separation based upon the immunoaffinity technique. By using th...
An Analysis of Anatolian Human Femur Anthropometry
Femur Anthropometry Anatolia Chalcolithic Age
2009/6/26
Femoral anthropometry from two different ages of Anatolian population groups was evaluated according to normal parametric measurements. Eleven femoral measurements with collo-diaphyseal angles were ob...
Genetic Polymorphisms in Steroid Hormone Metabolizing Enzymes in Human Breast Cancer
Breast cancer Genetic polymorphism Steroid hormone metabolism
2009/6/26
Epidemiologic studies indicate that most risk factors for breast cancer are related to reproductive and hormonal factors. The evaluation of associations between breast cancer risk and genetic polymorp...
Detection of Human Cytomegalovirus Gene in Formalin-Fixed Paraffin-Embedded Fetal Tissues
Diagnostic application of the ligation-dependant polymerase chain reaction
2009/6/26
Cytomegalovirus (CMV) is an opportunistic pathogen that can cause infection at any time during the course of a lifetime and constitutes an important cause of intrauterine infection and death in newbor...
Distribution of Different Fibronectin Isoforms in the Extracellular Matrices of Human Term Placenta
fibronectin human placenta extracellular matrix pregnancy
2009/6/25
Fibronectins are considered to be molecules that play important roles in cell migration and cell differentiation. Fibronectin molecules are thought to influence the differentiation of trophoblasts and...
Vitamin D Dependent Down Regulation of Arf1 Gene in Human Leukemia Cell Line HL-60
Gene expression ARF1 Vitamin D Real-Time RT-PCR
2009/6/24
ADP-ribosylation factor 1 (ARF1) is a gene belonging to the ARF gene family. ARF genes, small guanine nucleotide-binding proteins, constitute a family of the RAS superfamily. This superfamily function...
Recovery of 1-Chloro-2,4-dinitrobenzene Detoxification by N-acetyl- L-cysteine in Glutathione Predepleted Human Erythrocytes
GSH NAC Erythrocytes Xenobiotics Detoxification Free-SH
2009/6/23
Glutathione is an important thiol-containing compound involved in the detoxification process in erythrocytes. Its thiol group reacts with a variety of xenobiotics in a glutathione S-transferase cataly...