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Raising Standards in Semen Analysis：Professional and Personal Responsibility
Semen Analysis Personal Responsibility
2008/12/22
Studies by Keel (2004) and Brazil (Brazil et al, 2004a,b) have once again reminded us that quality control is the cornerstone of the andrology laboratory. To put this in context, numerous reports docu...
Enkephalin-Degrading Enzymes in Normal and Subfertile Human Semen
Opioid peptide peptidase seminal fraction sperm motility sperm death
2008/12/22
Opioid peptides have been reported to have important functions in human reproduction. Indeed, very high concentrations of enkephalins and their degrading enzymes have been reported in human semen. I...
Quality Control of Laboratory Methods for Semen Evaluation in a Multicenter Research Study
Sperm concentration semem volume sperm motility quality control observer variation precision
2008/12/22
Rigorously standardized laboratory protocols and strict quality control (QC) are essential for meaningful comparisons between semen quality data from multiple sites. We describe our experience wit...
Standardized Methods for Semen Evaluation in a Multicenter Research Study
Sperm concentration semen volume sperm motility quality control observer variation precision
2008/12/22
Semen evaluation methodology is complex and difficult to standardize. Rigorously standardized laboratory protocols and strict quality control (QC) are essential for meaningful comparison of data fro...
Semen Characteristics After Overnight Shipping：Preservation of Sperm Concentrations, HspA2 Ratios, CK Activity, Cytoplasmic Retention, Chromatin Maturity, DNA Integrity, and Sperm Shape
Reproductive toxicity male infertility cytoplasmic and nuclear biomarkers
2008/12/22
We tested several approaches that can be used to preserve sperm attributes and the objective biochemical markers of sperm maturity and function for assessment in a remote centralized laboratory afte...
A Flow Cytometric Method for Rapid Determination of Sperm Concentration and Viability in Mammalian and Avian Semen
Cytometry spermatozoa count vitality precision accuracy
2008/12/22
A new flow cytometric method has been developed to rapidly determine sperm concentration and viability in semen from bulls and boars. Sperm viability was determined on the basis of staining with SYB...
Cryopreservation of Ram Semen Facilitates Sperm DNA Damage: Relationship Between Sperm Andrological Parameters and the Sperm Chromatin Structure Assay
Artificial insemination semen quality motility chlortetracycline viability SCSA
2008/12/22
We hypothesized that cryopreservation and incubation in conditions that mimic the female genital tract following insemination increases the susceptibility of ram sperm DNA to denaturation. Ram spe...
Melatonin Administration Alters Semen Quality in Normal Men
Melatonin Administration Semen Quality Normal Men
2008/12/22
The publication by Luboshitzky et al (2002) concerning the effects of melatonin on human sperm quality deserves strong critique for a number of reasons. The authors performed a double-blind crossove...
Semen Quality of Men With Asymptomatic Chlamydial Infection
Chlamydia trachomatis infertility
2008/12/22
We have shown previously that the in vitro exposure of spermatozoa to elementary bodies (EBs) of Chlamydia trachomatis can lead to sperm death over a number of hours of incubation. As such, we hav...
High Levels of Sperm DNA Denaturation as the Sole Semen Abnormality in a Patient After Chemotherapy for Testis Cancer
Sperm DNA Denaturation Sole Semen Abnormality Patient Chemotherapy Testis Cancer
2008/12/19
Systemic chemotherapy for the treatment of cancer is known to adversely affect male fertility potential. Sperm DNA damage is reported after cancer chemotherapy and is associated with semen 0abnormalit...
Grace Centola writes regarding the performance of the semen analysis. Two labs have recently been cited by inspectors (NYS and CLIA inspectors) because sperm counts and motilities were not being don...
Evaluation of Chromosome Breakage and DNA Integrity in Sperm：An Investigation of Remote Semen Collection Conditions
Snap-freezing sperm-FISH TUNEL neutral comet assay
2008/12/19
Collection of ejaculated semen at a remote site (outside of the laboratory) would facilitate participation rates and geographic diversity in reproductive epidemiology studies. Our study addressed ...
Response：Seasonal Variation and Age-Related Changes in Human Semen Parameters
Seasonal Variation Age-Related Changes Human Semen Parameters
2008/12/19
We thank Drs. Mendeluk and Curi for their letter regarding our recently published article, "Seasonal Variation and Age-Related Changes in Human Semen Parameters." It is interesting that they also fo...
Re：Seasonal Variation and Age-Related Changes in Human Semen Parameters
Seasonal Variation Age-Related Changes Human Semen Parameters
2008/12/19
The study of seasonal variation in human semen parameters has, in our opinion, two different aspects—individual and demographic. The first refers to the consideration of each patient by an evaluatio...
Development of an Inexpensive Artificial Vagina for Semen Collection from Rabbits
Semen collection ejaculate device
2008/12/19
The rabbit is well suited for infertility research because of the animal's size, relatively low cost, and accessibility of the genitalia. There are several studies reporting sperm count and motility...