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以辣根过氧化物酶(horseradish peroxidase,HRP)为目标蛋白,N-异丙基丙烯酰胺(NIPAM)为单体,采用原位沉淀聚合法,在37 ℃,NIPAM和HRP质量比为6∶1的条件下,制备了粒径大小为13.7 nm,zeta电位是(-3.7± 0.3) mV的温度响应型辣根过氧化物酶纳米胶囊(nHRP).采用基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOF MS)、透射电镜(T...
Synthesis and Properties of Copolymer of Gallic Acid and Sulfamic Acid Catalyzing by HRP/ H2O2
Enzyme Catalytic Gallic Acid Horseradish Peroxidase Polymerization
2011/11/23
A phenolic copolymer (GASA) was synthesized from gallic acid (GA) and sulfamic acid (SAA) using horseradish peroxides (HRP)/H2O2 as catalyst. The effect of the main factors on yield of the copolymer w...
Preparation and Properties of Copolymer of Methacrylic Acid and Acrylamide onto Degraded Chitosan Initiated by HRP/H2O2/ACAC
Chitosan (CS) Graft Copolymerisation Horseradish Peroxidase Tanage
2011/12/14
The chitosan was degraded by hydrochloric acid and the effects of concentration of chitosan, amount of hydrochloric acid, reaction temperature and reaction time on the viscosity of chitosan were inves...
Synthesis and Properties of Phenolic Syntan with HRP Catalysis
Enzymatic Catalytic Polymerization Gallic Acid Horseradish Peroxides
2011/11/23
A phenolic syntan GA-SHBS had been synthesized by radical copolymerization of gallic acid (GA) and sodium 4-hydroxybenzenesulfonate (SHBS) using horseradish peroxides (HRP)/H2O2 as catalyst. The effec...
Preparation and Properties of Graft Copolymer of Phenols and Starch with HRP Catalysis
Degraded Starch Graft Copolymer Horseradish Peroxidase Synthetic Tanning Agent
2011/12/15
A graft copolymer was synthesized by means of radical copolymerization of degraded starch, phloroglucinol(PG) and sulfanilic acid (SA) and the reaction was catalyzed by horseradishperoxidase (HRP)/H2O...
Preparation of Grafting Starch by HRP Catalyst and it’s Application in Leather Tanning Process
Grafting Starch HRP Leather Tannage
2011/12/14
Two grafting starch was obtained by grafting copolymerization of two synthetical vinyl monomers using HRP/H2O2 as catalyst. The structure of vinyl monomers was verified by 1H-NMR.The grafting polymer ...
Preparation and Properties of Graft Copolymer of Phenols and Starch with HRP Catalysis
Degraded Starch Graft Copolymer Horseradish Peroxidase Synthetic Tanning Agent
2011/12/15
A graft copolymer was synthesized by means of radical copolymerization of degraded starch, phloroglucinol(PG) and sulfanilic acid (SA) and the reaction was catalyzed by horseradishperoxidase (HRP)/H2O...
Study on Preparation and Properties of Starch and 4-Phenolsuflonate Graft Copolymer with HRP Catalysis
4-Phenolsuflonate Graft Copolymer Horseradish Peroxidase Starch Synthetic Tannin
2011/12/14
Degraded starch was reacted with 4-phenolsuflonate (PHS) in water in the presence of horseradish peroxidase (HRP) catalyst/H2O2/acetylacetone (ACAC) to give starch and PHS graft copolymers. The struct...
用吸附的方法将辣根过氧化物酶(HRP)固定到三维笼状介孔分子筛FDU-12中, 傅立叶变换红外光谱(FTIR)和电化学交流阻抗谱结果表明, 固定后的HRP没有变性, 并表现出良好的直接电化学性质, 其式量电位(E0')为-0.325 V, 在40-300 mV·s-1范围内, 它不随扫描速率变化而变化. 电化学反应速率常数(ks)为1.200 s-1. 固定后的HRP对H2O2有稳定的电催化活性,...
超痕量HRP的邻苯二胺微粒瑞利散射光谱分析新方法及其应用
邻苯二胺 辣根过氧化酶 过氧化氢 瑞利散射光谱法
2009/11/16
在pH 4.8 的乙酸盐缓冲溶液中, 邻苯二胺(OPD)形成粒径约380 nm的微粒, 在392, 420, 445, 484和507 nm处有5个较强的Rayleigh散射峰. 辣根过氧化酶(HRP)催化H2O2氧化邻苯二胺生成黄色的2,3-二氨基吩嗪产物, 反应体系在420, 445和484 nm处的Rayleigh散射光信号显著减弱. 在最佳条件下, HRP浓度在8.3×10-12～4.17...
家兔肠系膜上神经节注射HRP后脊神经节内标记细胞的节段分布
脊神经节 辣根过氧化酶 家兔 肠系膜上神经节
2008/3/17
将ＨＲＰ（ｈｏｒｓｅｒａｄｉｓｈ ｐｅｒｏｘｉｄａｓｅ，辣根过氧化物酶）注入７只家兔肠系膜上神经节内，观察逆行标记神经元胞体在脊神经节内的节段性分布。结果显示，标记细胞见于双侧Ｔ ５（胸５）－Ｌ ３ （腰３）脊神经节内，较集中地分布于Ｔ ８ －Ｔ １２ 节段，高峰在Ｔ ９ 和Ｔ １０ 。两侧脊神经节内的标记细胞数及节段分布模式无显著差异。标记细胞散布于脊神经节各部，大都是５０μｍ以下的中，小型细...
植物病原细菌的hrp基因
hrp基因 植物病原细菌 过敏性反应 harpin蛋白
2008/1/30
摘要hrp基因存在于4类革兰氏阴性植物病原细菌中，决定病原细菌对寄主植物致病性和诱导非寄主及抗病植物过敏性反应。本文从hrp基因族，hrp基因avr基因的关系，hrp基因的编码产物harpin蛋白，hrp基因调控与功能以及hrp基因参与植物-细菌互作等几个方面，较为详细地阐述了当前植物病原细菌hrp基因的研究状况，并分析了hrp基因未来研究的趋势。
摘要Erwinia carotovora subsp. carotovora CSDS001菌株具有可直接诱导烟草过敏反应特征, 从构建的CSDS001菌株基因组文库, 鉴定、克隆到hrpNCSDS001基因, GenBank登录号AY939927; 构建的重组hrpNCSDS001基因工程菌株经IPTG诱导培养, 获得的高效表达HarpinCSDS001蛋白, 可诱导烟草发生过敏反应。30 mg...
将辣根过氧化物酶(Horseradish peroxidase；HRP)分别电泳入大灰褐蝙蝠(Eptesfeus luscus)和小灰褐蝙蝠(Myotis lucifugus)的小脑半球和蚓部对超声刺激有反应的记录点，以研究传递声信息到小脑去的有关神经结构。结果表明：小脑接受的主要声信息传入途径，是经过脑桥中转的，小脑也接受直接来自蜗核和上橄榄复合体的传入投射。此外，在下橄榄核和前庭核也发现标记细...
摘要 本文将辣根过氧化物酶(HRP)催化过氧化氢氧化KI生成I~2的反应与Luminol-I~2的化学发光反应相偶合,提出了测定HRP及其抗原、抗体标记物的高灵敏度的化学发光法。检测下限为7pg,线性范围为10-6000pg。通常使用的HRP直接催化Luminol-H~2O~2化学发光法灵敏度高10-100倍;克服了固相吸附法直接测定HRP标记物的缺陷,提高了测定的选择性。文中还对该偶合反应的机理...