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通过挖掘控制重要农艺性状的基因以提高农作物产量是育种学家一直关注的问题。然而由于农作物遗传背景复杂且基因组庞大, 该过程往往步骤繁琐, 耗时长且成本较高。Mapping-by-sequencing (利用高通量测序进行基因定位, MBS)利用二代测序技术和遗传分离群体混池建库分析的方法成功解决了上述问题, 加快了农作物重要农艺性状的基因定位同时也降低了研究成本。该方法已经成功应用于重要农艺性状相关...
Intron sequences from the elongation factor-1a (EF1a) gene from the marine shrimp Penaeus vannamei reveal extensive variation even among inbred populations of hatchery-raised shrimp. Among 44 indivi...
High-throughput sequencing technologies are currently revolutionizing the field of biology and medicine, yet bioinformatic challenges in analysing very large data sets have slowed the adoption o...
Species invasions cause significant ecological and economic damage, and genetic information is important to understanding and managing invasive species. In the ocean, many invasive species have...
Marbling is an important trait regarding the quality of beef. Analysis of beef cattle transcriptome and its expression profile data are essential to extend the genetic information resources and would ...
MicroRNAs (miRNAs) are small, endogenous RNAs that play important regulatory roles in development and stress response in plants by negatively regulating gene expression post-transcriptionally.Medicago...
Ethylene is one ofthe classical plant hormones with a diverse function in plant growth and development. Root elongation is sensitive to ethylene such that treatments with ethylene and the ethylene pre...
MicroRNAs (miRNAs) play important roles in response of plants to biotic and abiotic stresses. Aluminum (Al) toxicity is a major factor limiting plant growth in acidic soils. However, there has been li...
Backg round: MicroRNAs (miRNAs) are small, endogenous RNAs that play important regulatory roles in development and stress response in plants by negatively affecting gene expression post-transcriptiona...
The development of new methods to carry out gene transfer has many benefits to several fields, such as gene therapy, agriculture and animal health. The newly established lentiviral vector systems fur...
Rapid sequencing of individual human genome is prerequisite to genomic medicine, where diseases will be prevented by preemptive cures. Quantum-mechanical tunneling through single-stranded DNA in a sol...
Faster sequencing of DNA holds enormous potential for biology and medicine, particularly for personalized diagnosis and customized treatment based on each individual's genomic makeup. At present howev...
The dearth of general-use (i.e. quasi-‘universal’) primer sets for the amplification of single-copy nuclear DNA (scnDNA) loci, combined with the availability and ease of use of mitochondrial (mtDNA...
A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif (ADAMTS1) plays a critical role in follicular rupture and represents a major advance in the proteolytic events...
The Human Genome Project is the largest biological project ever undertaken. The project goals are to: Identify variations in the human genetic code that cause disease; Determine how individual genes p...

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