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Tomato's wild ancestor is a genomic reservoir for plant breeders（图）
Tomato wild ancesto genomic reservoir plant breeders
2020/12/11
Thousands of years ago, people in South America began domesticating Solanum pimpinellifolium, a weedy plant with small, intensely flavored fruit. Over time, the plant evolved into S. lycoper...
Congruence of RAPD and ISSR Markers for Evaluation of Genomic
Podophyllum hexandrum RAPD ISSR RAPD+ISSR Genetic Diversity
2010/3/17
Twenty eight populations of Podophyllum hexandrum Royle were selected to study genetic relationship using RAPD and ISSR markers from north-western Himalayas, Himachal Pradesh, India. Nineteen RAPD pri...
改良CTAB法用于多年生植物组织基因组DNA的大量提取--An Efficient Macro-method of Genomic DNA Isolation from Actinidia chinensis Leaves
猕猴桃 DNA 提取 Southern杂交 Actinidia genomic DNA isolation Southern blot
2007/12/5
根据多年生植物组织富含多酚、多糖的具体特性，对现有的DNA提取方法进行了改进。通过增加提取缓冲液中b-巯基乙醇用量，简化氯仿/异戊醇抽提液步骤，改用经-20℃预冷异丙醇沉淀DNA等，对CTAB法加以改进。改进后方法具有以下优点：（1）获得的DNA质量良好，提取过程无明显的DNA降解，基本上排除了多酚物质的干扰；（2）用获得的DNA进行Southern杂交，可得到理想的杂交信号，可满足相关的分子研究...