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We generally think of inheritance as the genetic transfer from parent to offspring and that evolution moves toward greater complexity. But there are other ways that genes are transferred between organ...
Genetic analysis of two mutants altered with respect to formation of polyhydroxyalkanoic acid and identification of putative RNA helicase, nuclease and gas vesicle genes in {\it Bacillus megaterium\/}
Genetic analysis of two mutants altered with respect to formation of polyhydroxyalkanoic acid and identification of putative RNA helicase nuclease and gas vesicle genes in Bacillus megaterium
2015/1/28
To study polyhydroxyalkanoate (PHA) accumulation and PHA regulation in Bacillus megaterium, transposon mutagenesis with Tn917-LTV1 was carried out and seventy two mutants that produce more or less PHA...
Researchers from North Carolina State University have developed a technique that co-opts an immune system already present in bacteria and archaea to turn off specific genes or sets of genes – creating...
鉴定痢疾杆菌毒力基因的SW480细胞模型构建Construction of SW480 Cell Model Identifying Shigella Virulent Genes
SW480 细胞模型 痢疾杆菌 信号标签诱变技术 构建
2008/1/8
摘要
信号标签诱变技术（STM）是一种在体内高通量筛选病原体毒力基因的新方法，在应用时的一个先决条件是要建立合适的体内筛选系统。为将该技术应用于福氏痢疾杆菌，我们使用三个福氏痢疾杆菌菌株进行了预试验：通过同源重组构建而成的带有氯霉素抗性且aroA和virG基因失活的突变株RC426；因在侵袭质粒上自发缺失3个基因座(ipaBCDA, invA 和 virG)的另一减毒突变株T32，其曾被用作福氏...