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The aim of this thesis is to develop a biological database mining tool that incorporates mining of various publicly available heterogeneous databases and provides researchers with a reporting and visu...
PURIFICATION OF SIMPL ANTIBODY AND IMMUNOFLUORESCENCE OF SIMPL SUB-CELLULAR LOCALIZATION IN RESPONSE TO TNFα- AND IL-1
SIMPL Transcriptional Co-Activator NF-kB
2015/5/25
SIMPL is a transcriptional co-activator that alters the activity of transcription factor, NF-κB. In response to pathogens, cytokines such as Interleukin-1 (IL-1) and Tumor Necrosis Factor (TNF) signal...
摘要
为了用绿色荧光蛋白标记观察人类无精症相关基因ZNF230在Cos7细胞中的蛋白质表达及定位，用PCR方法扩增得到突变的人和小鼠mt-ZNF230和mt-znf230基因，使其3′端的终止密码TGA突变为TGG，并装入T-载体，双酶切后通过定向克隆将其与真核表达载体pEGFP-N1的绿色荧光蛋白（green fluorescence protein，GFP）基因融合，构建了ZNF230—荧光...