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Kinetics of hydrogen peroxide generated from live and dead ram spermatozoa and the effects of catalase and oxidase substrates addition
reactive oxygen species ram antioxidant
2016/2/23
The generation of hydrogen peroxide (H2O2) by ram spermatozoa (spz) was measured using a flurometric assay with 10-acetyl-3,7-dihydroxyphenoxazine agent as a probe for H2O2 detection. The kinetics of ...
Characterisation of polyphenol oxidase and peroxidase and the role in browning of loquat fruit
Eriobotrya japonica PPO POD
2015/3/30
Polyphenol oxidase (PPO) and peroxidase (POD) were extracted from a new loquat fruit cultivar (Ninghaibai) and characterised using reliable spectrophotometric methods. In both cases, the optimum pH fo...
Characterisation of polyphenol oxidase from Melissa officinalis L. subsp. officinalis (lemon balm)
lemon balm protein enzyme kinetics inhibition
2014/2/24
Polyphenol oxidase (PPO) from Melissa officinalis L. subsp. officinalis (lemon balm) was partially purified by ammonium sulphate precipitation and dialysis; and then it was characterised in detail in ...
Influence of Developmental Stage on Activities of Polyphenol Oxidase, Internal Characteristics and Colour of Lettuce cv. Grand Rapids
Developmental Stage Polyphenol Oxidase
2016/5/31
An analyzing study to evaluate the relationship between Polyphenol Oxidase (PPO) activity, internal qualities and degree of browning was conducted on lettuce var. Grand Rapids in order to understand t...
Characterization of Glucose Oxidase from Penicillium notatum
Penicillium notatum glucose oxidase thermostability
2010/2/1
In the present study glucose oxidase (GOD) has been isolated from a culture filtrate of Penicillium notatum. The enzyme was purified by ammonium sulphate precipitation, diethylaminoethyl (DEAE) cellul...
Variation of Total Polyphenol and Polyphenol Oxidase Activity during Maturation of Mango Fruit (Mangifera indica L. ‘Irwin’) Cultured in a Plastic House
‘Irwin’ mango fruit maturation of mango variation of total polyphenol
2009/7/8
Variation of total polyphenol (TPP) and polyphenol oxidase activity (PPO) was investigated in ‘Irwin’ mango fruit in various color stages of development: green, purple, purplish red and red. Fruits us...
Effects of Polyphenol Oxidase Activity in Wheat Flour on Protein-Bound 5-S-Cysteinyldopa Formation in Gluten
5-S-cysteinyl-3 4-dihydroxyphenylalanine (5-S-cysteinyldopa) polyphenol oxidase
2009/7/8
The effects of polyphenol oxidase (PPO) activity in wheat flour on the formation of protein-bound 5-S-cysteinyl-3, 4-di-hydroxyphenylalanine (5-S-cysteinyldopa) in gluten have been studied. The wheat ...
Effects of Sucrose Palmitic Acid Ester Coating on the 1-Aminocyclopropane-1-Carboxylate (ACC) Oxidase Activity in the Peel and Pulp of Ripening Banana Treated with Ethylene
Banana ripening sucrose palmitic acid ester
2009/7/7
Bananas were coated with 2% sucrose palmitic acid ester after treatment with 100 ppm ethylene for 12 h and stored at 20°C. The use of 2% sucrose palmitic acid ester coating significantly reduced weigh...
Modified Alcohol Oxidase Method Used to Determine the Degree of Pectin Methylesterification
methylesterification spectrophotometry determination
2009/6/18
The degree of methylesterification of pectin was measured using a modified alcohol oxidase method to determine the methanol liberated from pectin by saponification. When the alcohol oxidase method was...
Comparison of Soluble and Plastidal Polyphenol Oxidase in Mature Apples
polyphenol oxidase plastid subcellular localization
2009/6/17
Polyphenol oxidase (PPO) is transported to plastid after translation. However, we often detect PPO activity in a soluble fraction of apple. Here we examined the location of PPO in retail apples. PPO a...
Purification and Characterization of Wheat（Triticum aestivum）Polyphenol Oxidase
wheat polyphenol oxidase purification
2009/6/10
Polyphenol oxidase was purified to homogeneity from Japanese wheat (Triticum aestivum cv. Tohoku 198) bran, using 4-methylcatechol as a substrate. The enzyme was purified 1670-fold with a recovery of ...
Banana Polyphenol Oxidase: Occurrence and Change of Polyphenol Oxidase Activity in Some Banana Cultivars during Fruit Development
polyphenol oxidase tyrosynase development period
2009/5/31
Occurrence of polyphenol oxidase (PPO, o-diphenol: oxygen oxidoreductase, EC.1.10.3.1) in the fruits of nine cultivars of banana (Musa spp.) commercially cultivated in China was investigated. All bana...
Purification and Characterization of Polyphenol Oxidase from Edible Yam（Dioscorea opposita Thunb.）
polyphenol oxidase edible yam dopamine oxidase
2009/5/31
Soluble polyphenol oxidase of edible yam (Dioscorea opposita Thunb.) was purified approximately 203-fold with a recovery rate of 15% by ammonium sulfate fractionation, ion exchange chromatography, hyd...
Membrane Microfiltration Fermentation of Glucose Oxidase with Cell Recycling
glucose oxidase membrane microfiltration fermentation cell recycling
2009/4/28
Membrane microfiltration fermentation (MMF) with cell recycling was successfully applied to the production of glucose oxidase (GOD). A plate microfiltration module was found suitable for such purpos...
A New Oxidase-Peroxidase Kit for Ethanol Assays in Alcoholic Beverages
ethanol enzymatic analysis alcoholic beverages
2009/3/20
The paper presents analytical parameters of an enzymatic method for alcohol assay in alcoholic beverages by the use of a new kit based on alcohol oxidase and peroxidase catalyzed reactions (AOP). Sens...