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Down regulation of miR-203 in radiation- induced thymic lymphoma promoted cells proliferation and inhibited apoptosis
TANK-binding kinase 1 (TBK1) SLUG (SNAI2) Cyclin D1 (CCND1)
2015/11/24
To investigate the role of miR-203 in radiation-induced thymic lymphoma (RITL).Methods A 60Co irradiator was used for total-body irradiation. MicroRNAs(miRNAs) level was assayed by qRT-PCR. Cell proli...
构建含有人源microRNA-203（miR-203）的慢病毒表达载体并制备重组慢病毒, 感染人脐静脉内皮细胞系（HUVEC-12）, 观察其对细胞增殖和迁移的影响。方法 使用PCR方法克隆miR-203前体分子的DNA片段, 构建并包装过表达miR-203的重组慢病毒。利用慢病毒感染系统建立稳定过表达miR-203的人脐静脉内皮细胞株（HUVEC-Lv-miR-203）。实时荧光定量PCR（qR...