搜索结果: 1-3 共查到“医学细胞生物学 GST”相关记录3条 . 查询时间(0.046 秒)
构建pGEX-4T-1/SUMO3重组表达质粒，并在大肠杆菌中表达和纯化出类泛素化修饰蛋白SUMO3/GST融合蛋白。方法：采用PCR技术利用pcDNA3.1-SUMO3质粒为模板，扩增出SUMO3全长编码序列（312 bp），并将其重组于谷胱甘肽硫转移酶（GST）融合蛋白表达质粒pGEX-4T-1中，酶切、测序鉴定获得重组质粒。将重组质粒转化E.coli BL21，异丙基β-D-硫代半乳糖苷（I...
重组蛋白rhTRAIL55-281与GST-rhTRAIL55-281有肝细胞毒性
重组蛋白 rhTRAIL 55-281 GST-rhTRAIL 55-281 抗肿瘤药物 肝细胞 毒性 细胞凋亡
2009/8/12
目的: 检测重组蛋白rhTRAIL55-281与GST-rhTRAIL55-281的对肝细胞的毒性, 以确定其是否可以开发为抗肿瘤药物. 方法: 采用胰酶消化与机械分离结合的方法, 获取原代成人肝细胞和胎肝细胞. 用Factor-Xa切除重组蛋白GST-rhTRAIL55-281的GST标签获取rhTRAIL55-281蛋白, 然后分别利用rhTRAIL55-281与GST-rhTRAIL55-2...
Technical Approach to Generate Polyclonal Antibodies Against Bacterially Expressed GST-PYK- C: Evaluation of Cross-Reaction and Recognition in Some Mammalian and Yeast Cell Lysates
pyk-2 Central Nervous System Molecular Cloning Gene Fusion Bacterial Proteins Antibody Formation Antibody Specificity Immunochemistry Immunoblot-ting
2009/7/1
Pyk-2 is a protein tyrosine kinase, highly expressed in human hippocampus, dentate gyrus and olfactory bulb. In this study, polyclonal antibodies against the C-terminal of pyk-2, starting from Pro-759...