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Relationships between computerized measurements of motion of frozen-thawed bull spermatozoa and fertility
computerized measurements motion frozen-thawed bull spermatozoa fertility
2009/2/23
A computerized system (CellSoft, CRYO Resources, Ltd.) was validated using video tapes of frozen-thawed bull spermatozoa diluted in filtered (0.2 micron) egg yolk-citrate extender (8 X 10(6) spermat...
Lectin-binding pattern of bull testis and epididymis
Lectin-binding pattern bull testis epididymis
2009/2/23
Seven rhodamine-conjugated lectins (PNA, RCA I, SBA, Con A, WGA, UEA I, DBA) were used to study the distribution of glycoproteins in the testis and epididymis of immature, juvenile, and adult bulls....
Specific binding of the glycosaminoglycan 3H-heparin to bull, monkey, and rabbit spermatozoa in vitro
sperm acrosome reaction glycosaminoglycans
2009/2/20
In Vitro binding and some binding parameters of the glycosaminoglycan heparin to viable epididymal or ejaculated bull spermatozoa, ejaculated rabbit spermatozoa, and frozen-thawed rhesus monkey sper...
The effect of exogenous follicle stimulating hormone treatment on anterior pituitary and testicular function in the prepubertal bull
follicle stimulating hormone luteinizing hormone testosterone prepubertal bull
2009/2/20
The effect of exogenous follicle stimulating hormone (FSH) on testicular function was studied in twenty 4-month-old Holstein bulls during two seasons (ten in late winter and ten in summer). Five mg ...
Disappearance of Inhibin-like Activity in Bull Seminal Plasma Following Castration
inhibin seminal plasma castration
2009/2/19
Ejaculates from normal and castrated bulls were evaluated for the presence of inhibin-like activity. Inhibin activity found in the seminal plasma of normal bulls became undetectable after castrati...
Reproductive tract secretions and bull spermatozoa contain different clusterin isoforms that cluster cells and inhibit complement-induced cytolysis
Epididymal fluid rete testis fluid L-cells
2009/2/18
Clusterin from bull rete testis fluid (RTF), cauda epididymal fluid (CEF), and octyl-beta-D-glucopyranoside extract of cauda epididymal sperm (CES) was identified and characterized using monoclonal ...
Exposure of thawed frozen bull sperm to a synthetic peptide before artificial insemination increases fertility
Enhancement of fertility bovine sperm prosaposin
2009/2/18
We evaluated the effect on fertility of in vitro exposure of thawed frozen bull sperm to synthetic FertPlus peptide prior to artificial insemination (AI). The peptide represented a 60-amino acid s...
Exposure of human, boar, or bull sperm to a synthetic peptide increases binding to an egg-membrane substrate
human boar bull sperm synthetic peptide increases egg-membrane substrate
2009/2/18
We evaluated the effects of in vitro exposure of sperm to synthetic FertPlus peptide, which represents a 60-amino acid sequence within rat prosaposin, using a microwell sperm-binding assay (SBA), ...
Artificial insemination using cryopreserved semen is a common management tool of the contemporary livestock producer. However, cryopreservation is detrimental to sperm function and fertility, kill...
Use of Hoechst 33342 stain to evaluate live fresh and frozen bull sperm by computer-assisted analysis
Sperm motility DNA ultraviolet illumination
2009/2/17
The objective of this research was to investigate possible procedures for evaluating living bull sperm stained with Hoechst 33342 while in a simple medium and in commonly used complex egg yolk-glyce...
Anti-bull sperm monoclonal antibodies：II. Binding changes during capacitation and influence on sperm-zona interactions in vitro
Bull sperm sperm-zona binding capacitation
2009/2/16
Anti-bull sperm monoclonal antibodies (mAbs), generated against intra-acrosomal and surface antigens, were evaluated for their functional significance. In experiment I, the influence of mAbs on the bo...
Anti-bull sperm monoclonal antibodies：I. Identification of major antigenic domains of bull sperm and manifestation of interspecies cross-reactivity
Anti-sperm antibodies antigen localization conserved
2009/2/16
The overall objective of this series of experiments is to generate immunological markers that may elucidate bull sperm surface changes in vitro. Here we report the initial experiments of the study, ...
Media and dilution procedures tested to minimize handling effects on human, rabbit, and bull sperm for computer-assisted sperm analysis（CASA）
CASA holding time hyperactivation TALP PBS
2009/2/16
Proper handling of semen prior to computer-assisted sperm analysis (CASA) is critical if the analysis is to be representative of the fresh sample. The effects of diluting medium or dilution and ho...
A comparison of critical osmolality and hydraulic conductivity and its activation energy in fowl and bull spermatozoa
Water permeability plasma membrane flow cytometry
2009/2/10
Measurements were made of critical osmolality, the osmolality at which 50% of the cells are lysed, and of the permeation time, the time taken to lyse 50% of the cells in an osmotic solution lower ...
Capacitation and Induction of the Acrosome Reaction in Bull Spermatozoa With Norepinephrine
Catecholamines bovine
2008/12/30
Identification of norepinephrine (NE) within the microenvironment of the bovine oviduct suggests a potential role for catecholamines in the events surrounding fertilization. Previous studies have s...