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The effect of selective destruction and regeneration of rat Leydig cells on the intratesticular distribution of testosterone and morphology of the seminiferous epithelium
selective destruction regeneration rat Leydig cells intratesticular distribution
2009/2/23
This study was designed to explore the relationship between the intratesticular distribution of testosterone and spermatogenesis by completely destroying the Leydig cells of mature male rats with in...
Identification and distribution of Pz-peptidases A and B in human semen
Identification distribution Pz-peptidases A and B human semen
2009/2/23
Human semen was fractionated into fluid, particle and spermatozoal constituents using Percoll density gradient centrifugation followed by additional separation steps. All of the fractions isolated p...
Rat Testicular Phospholipase A2 Activity：pH Optima, Its Cellular and Subcellular Distribution in the Gonad, and Some Factors That May Modulate Its Activity
rat testicular phospholipase A2 activity pH optima localization control
2009/2/19
A sensitive radiometric assay was developed for detecting phospholipase A1 and A2 (PLA1 and A2) activity. Four to five times more PLA2 activity was observed in rat testes than PLA1 activity. Two PLA...
Distribution of mouse sulfated glycoprotein-1（prosaposin）in the testis and other tissues
Immunoperoxidase staining immunogold labeling
2009/2/17
Mouse sulfated glycoprotein-1 (SGP-1) is the homologue of rat SGP-1 and human prosaposin. Rat SGP-1 is one of the major secretory products of rat Sertoli cells in culture. Human prosaposin is the pr...
Regional distribution of 5alpha-reductase type 1 and type 2 mRNA along the nonhuman primate (Macaca fascicularis) epididymis
Cynomolgus monkey RT-PCR gene expression
2009/2/17
The 5alpha-reduced metabolites of testosterone, including dihydrotestosterone, are considered the primary regulators of epididymal function. Two genes encode two 5alpha-reductase isozymes. We exam...
Apical and narrow cells are distinct cell types differing in their structure, distribution, and functions in the adult rat epididymis
Apical and narrow epithelial cells initial segment intermediate zone epididymis rat
2009/2/16
Apical and narrow cells of the initial segment and intermediate zone of the adult rat epididymis were glutaraldehyde fixed and Epon embedded for routine light (LM) and electron (EM) microscopic an...
Distribution and tissue expression of semenogelin I and II in man as demonstrated by in situ hybridization and immunocytochemistry
Semen spermatozoa fertilization Southern blot
2009/2/16
Semenogelin I and II (Sgl, Sgll) are two separate gene products of chromosome 20 with extensive (80%) identity in primary structure. They are mainly responsible for immediate gel formation of fres...
The distribution of connexin 43 is associated with the germ cell differentiation and with the modulation of the Sertoli cell junctional barrier in continual（guinea pig）and seasonal breeders'（mink）testes
Gap junction Cx43 Sertoli cell blood-testis barrier
2009/2/13
To test whether the gap junction protein connexin 43 (Cx43) is associated with germ cell differentiation and with the Sertoli cell junctional blood barrier, we recorded the temporal changes in its d...
GnRH-A induced arrest of spermiogenesis in rats is associated with altered androgen binding protein distribution in the testis and epididymis
GnRH-antagonist Sertoli cell spermatogenesis
2009/2/10
This study examines the effects of a potent gonadotropin releasing hormone (GnRH)-antagonist (GnRH-A, Ac-D[2] Nal1, 4-CL-D Phe2, D-Trp3, D-Arg6, D-Ala10) upon the distribution of androgen binding pr...
Non-poisson distribution of sperm from grandfathers in zona-free hamster ova
Penetrating sperm age Poisson distribution human
2009/2/7
Earlier reports indicated that sperm from 25% of patients from infertile couples, but not from normal or fertile donors, show deviations from the theoretical Poisson distribution of the number of sp...
Separation of ram spermatozoa bearing X and Y chromosome by centrifugal countercurrent distribution in an aqueous two-phase system
ram spermatozoa X and Y chromosome centrifugal countercurrent aqueous two-phase system
2008/12/25
The availability of reliable quantification techniques of X and Y chromosome-bearing spermatozoa in a given insemination dose would allow further approaches to their separation, which is a topic o...
Dynamic subcellular distribution of the DAZL protein is confined to primate male germ cells
Dynamic subcellular distribution DAZL protein primate male germ cells
2008/12/25
We have examined the location of the DAZL protein in fetal and adult rodents and human specimens and found that there is a shift from a predominantly nuclear to a predominantly cytoplasmic distribut...
Characterizing the Glycocalyx of Poultry Spermatozoa：I. Identification and Distribution of Carbohydrate Residues Using Flow Cytometry and Epifluorescence Microscopy
Turkey chicken semen glycoconjugate lectin
2008/12/24
The aim of the present work was to use a battery of lectins to 1) delineate the carbohydrate content of sperm glycocalyx in the turkey and chicken using flow cytometry analysis, and 2) evaluate the ...
Follicle-Stimulating Hormone Receptor Gene Haplotype Distribution in Normozoospermic and Azoospermic Men
Male infertility polymorphism SNP testis azoospermia
2008/12/23
The human follicle-stimulating hormone (FSH) receptor (FSHR) gene possesses single nucleotide polymorphisms (SNP) in exon 10, which influence serum FSH levels in women, but not in men. In the presen...
Particle Distribution in Low-Volume Capillary-Loaded Chambers
Spermatozoa concentration capillary slide hemacytometer Poiseuille Segre-Silberberg
2008/12/23
Accurate determination of sperm concentration in fluid suspension is a critical component in a semen analysis. Inaccurate estimations can lead to misinterpretation of the spermiogram and, in the c...