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Localization of tubulin and microtubules of in vivo fertilized rabbit oocytes
fertilization microtubules acrosome acrosin
2009/2/20
The presence of tubulin throughout what appears to be sperm penetration tunnels of in vivo fertilized rabbit oocytes was demonstrated by both immunofluorescence and transmission electron microscopy,...
Comparison of glycerol and a zwitter ion buffer system as cryoprotective media for human spermatozoa. Effect on motility, penetration of zona-free hamster oocytes, and acrosin/proacrosin
spermatozoa human cryopreservation
2009/2/20
This study compared the cryoprotective effect of glycerol with that of a zwitter ion buffer system (TESTCY). Spermatozoa that are cryopreserved in the presence of glycerol possess a somewhat highe...
Sperm motility enhancement by nitric oxide produced by the oocytes of fathead minnows, Pimephelas promelas
Fertilization CASA electron spin resonance oocyte
2009/2/18
The effects of nitric oxide (NO) on sperm motility were examined in the fathead minnow, Pimephelas promelas, using computer-assisted sperm analysis (CASA). The observed effects underscore the dual n...
The use of two fluorescent dyes to identify sperm in a competitive binding assay to oocytes
fluorescent dyes identify sperm competitive binding assay oocytes
2009/2/18
The relationship of most sperm laboratory assays to male fertility is inconsistent. Assays that measure traits required to fertilize oocytes are expected to have the most predictive value. A new ass...
Effect of bovine oviductal estrus-associated protein on the ability of sperm to capacitate and fertilize oocytes
Oviduct culture proteinsecretion oviductal fluid
2009/2/12
At estrus, an oviduct-specific, estrus-associated glycoprotein (EAP) of molecular weight 85-95,000, is secreted by the oviductal epithelium and found in cannula-derived bovine oviductal fluid (ODF). T...
First Steps in the Development of a Functional Assay for Human Sperm Using Pig Oocytes
Sperm functionality assay species specificity sperm-oocyte interaction heterologous ICSI
2008/12/24
The use of mammalian oocytes to assess human sperm functionality could be a helpful tool with potential applications in clinical and research programs. In an attempt to develop the pig model, the...
Does Seminal Plasma PSP-I/PSP-II Spermadhesin Modulate the Ability of Boar Spermatozoa to Penetrate Homologous Oocytes In Vitro?
Seminal plasma preservation sperm viability IVF pig
2008/12/22
Low concentration (0.15 mg per million of spermatozoa) of seminal plasma–derived PSP-I/PSP-II spermadhesin heterodimer is able to preserve the viability of highly extended boar spermatozoa. Whether ...
Lignosulfonic Acid Blocks In Vitro Fertilization of Macaque Oocytes When Sperm Are Treated Either Before or After Capacitation
Contraception fucoidin microbicide polysulfonation zona pellucida
2008/12/16
Lignin-derived macromolecules (LDMs) are biologically active compounds that affect a variety of cell-to-cell interactions including the inhibition of fertilization and embryo development in a number o...