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Quantification of bovine sperm separation by a swim-up method. Relationship to sperm motility, integrity of acrosomes, sperm migration in polyacrylamide gel and fertility
Quantification bovine sperm separation swim-up method
2009/2/23
The number of bovine spermatozoa separated in a swim-up procedure was quantified using an electronic cell counter. In an initial test of the swim-up procedure, non-frozen sperm samples with differen...
Effects of substrate and separation method on acrosin amidase measurements
Enzyme activity DL-BAPNA L-BAPNA swim-up
2009/2/16
The purpose of this study was to increase the accuracy and reproducibility of the acrosin amidase assay and to assess the effects of different methods of sperm isolation on total sperm acrosin act...
Subfertility in stallions is common, and methodologies are needed to increase the fertility in these animals. In other species, removal of the dead sperm from semen increases the quality and ferti...
Effects of caudal elevation on testicular function in rats. Separation of effects on spermatogenesis and steroidogenesis
Hind-limb unloading testicular function testosterone spermatogenesis microgravity rats
2009/2/10
A variety of biologic processes are perturbed when exposed to microgravity (space flight) for more than 7 days, including testicular function. Suspension of rats in a special harness (caudal elevati...
Separation of cryopreserved human semen using Sephadex columns, washing, or Percoll gradients
Sperm separation intrauterine insemination seminal plasma sublethal damage
2009/2/7
The following methods were evaluated for their ability to separate motile cryopreserved sperm from semen after thawing: single washing, Percoll separation followed by a single washing, and Sephad...
Separation of ram spermatozoa bearing X and Y chromosome by centrifugal countercurrent distribution in an aqueous two-phase system
ram spermatozoa X and Y chromosome centrifugal countercurrent aqueous two-phase system
2008/12/25
The availability of reliable quantification techniques of X and Y chromosome-bearing spermatozoa in a given insemination dose would allow further approaches to their separation, which is a topic o...