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scTRAIL在E.coli中的重组表达及抗肿瘤活性的研究
肿瘤坏死因子相关凋亡诱导配体 单链化 大肠埃希菌 重组表达 抗肿瘤活性
2020/8/10
重组表达人单链TRAIL（single-chain TRAIL，scTRAIL）及研究其在抗肿瘤中的作用。方法：通过重组PCR获得编码scTRAIL的基因序列，构建含编码scTRAIL基因的重组质粒，将重组表达质粒转化到大肠埃希菌(E.coli)BL21（DE3），通过IPTG诱导，破碎细菌，上清行Amylose Resin亲和层析初步纯化融合蛋白。通过MTT法检测融合蛋白对人胰腺胆管上皮细胞SW...
旨在分析探讨TLR5基因启动子区甲基化修饰对断奶仔猪F18大肠杆菌抗性的调控作用。本研究首先利用qPCR和Western blot检测分析了TLR5基因在F18大肠杆菌抗性型和敏感型苏太断奶仔猪小肠组织（十二指肠和空肠）中的差异表达，然后利用生物信息学分析和双荧光素酶报告系统检测确定TLR5基因核心启动子区、CpG岛及其作用元件，进而检测并分析TLR5基因启动子区甲基化修饰与TLR5基因在F18大...
旨在细胞水平上进一步探讨FUT2基因的功能及其在猪小肠上皮细胞受到F18大肠杆菌（Escherichia coli，E.coli）侵染时的调控机制，为提高仔猪对F18大肠杆菌病抵抗力的分子选育提供理论基础。运用Real-time PCR方法检测分析FUT2基因在大肠杆菌F18ab、F18ac感染和脂多糖（LPS）诱导小肠上皮细胞前后的mRNA表达差异，并采用Western blotting方法检测...
Molecular characterisation of Shiga toxin-producing Escherichia coli O157:H7 isolates from cattle in eastern Turkey
pulsed-field gel electrophoresis virulence genes bovine rectal swabs E. coli strains
2016/12/8
Shiga toxin-producing Escherichia coli (STEC) O157:H7 is an important foodborne pathogen worldwide. In Turkey, little is known about the molecular characteristics of STEC O157:H7 isolates. The aim of ...
Pneumatosis coli in a dog–a serial radiographic study:a case report
colon computed tomography dog emphysema
2016/8/2
A 12-year-old intact female poodle was presented with a history of an acute episode of tenesmus and passage of ribbon-shaped stools. Anaemia, leucocytosis, hypoalbuminaemia, hyperglycaemia, and elevat...
Antibacterial characteristics of orange pigment extracted from Monascus pigments against Escherichia coli
inhibition zone minimum inhibitory concentration antibacterial activity
2016/7/6
The antibacterial characteristics of orange pigment, which is one of the Monascus pigments, against Escherichia coli were investigated. Orange pigment exhibited strong antibacterial activity against E...
Antimicrobial effects of curcumin against L. monocytogenes,S.aureus,S.Typhimurium and E.coli O157:H7 pathogens in minced meat
curcumin antimicrobial effect foodborne pathogen minced meat
2016/6/3
The aim of this study was to determine the antimicrobial efficacy of curcumin, one of the active components of the Curcuma longa (turmeric) plant, against food pathogens in a minced meat medium. Salmo...
Effect of Polysaccharides from Acanthopanax senticosus on Intestinal Mucosal Barrier of Escherichia coli Lipopolysaccharide Challenged Mice
Herbal Extract Intestinal Barrier Inflammation Epidermal Growth Factor Pro-inflammatory Cytokines Metabolism
2016/5/20
To investigate the role of polysaccharide from Acanthopanax senticosus (ASPS) in preventing lipopolysaccharide (LPS)-induced intestinal injury, 18 mice (at 5 wk of age) were assigned to three groups w...
Effect of Two Doses of Different Zinc Sources (Inorganic vs. Chelated form) on the Epithelial Proliferative Activity and the Apoptotic Index of Intestinal Mucosa of Early-weaned Pigs Orally Challenged with E. coli K88
Weaning Pig Zinc Oxide Small Intestine Apoptosis Mitosis
2016/5/4
The effect of two doses of different sources of zinc, inorganic (zinc oxide) or chelated (zinc glutamate chelate), on morphology and turn-over of the small intestine was assessed in early-weaned pigs ...
Screening of antimicrobial resistance and molecular detection of fluoroquinolone resistance mechanisms in chicken faeces-derived Escherichia coli
antibiotic susceptibility E. coli rep-PCR
2016/2/23
This study was aimed at investigating the resistance to antimicrobial agents and to assess the predominant molecular mechanisms of fluoroquinolone resistance in faecal E. coli strains isolated from ch...
High-level Expression and Purification of Active Human FGF-2 in Escherichia coli by Codon and Culture Condition Optimization
Fibroblast Growth Factor 2 Codon Protein E. coli
2016/1/26
Background: Basic fibroblast growth factor (bFGF) is a member of a highly conserved superfamily of proteins that are involved in cell proliferation, differentiation, and migration.
Objectives: The ob...
High Frequency of Class 1 Integrons in Escherichia coli Isolated From Patients With Urinary Tract Infections in Yasuj, Iran
Integrons Urinary Tract Infections Multidrug Resistant Escherichia coli
2016/1/26
Background: Most urinary tract infections (UTI) are caused by Escherichia coli. Integrons have an important role in distributing antibiotic resistance genes among bacteria.
Objectives: The aim of thi...
Shiga Toxigenic Escherichia coli in Iranian Pediatric Patients With and Without Diarrhea: O-Serogroups, Virulence Factors and Antimicrobial Resistance Properties
Microbial Sensitivity Tests Virulence Factors Iran Shiga Toxigenic Escherichia coli
2015/11/9
Background: Shiga-toxigenic Escherichia coli is an important human pathogen cause of diarrhea, hemorrhagic colitis, hemolytic uremic syndrome and thrombotic thrombocytopenic purpura in humans is a sig...
Anti Microbial Resistance Profile of E. coli isolates From Tropical Free Range Chickens
E. coli Anti-microbial resistance Local fowl Turkey Roosters Cockerels Nigeria
2015/7/29
Normal intestinal flora of humans and animals constitute enormous reservoir of resistance genes for potentially pathogenic bacteria and may serve as major indictors of selection pressure exerted by an...
Assessing the Importance of Sand as a Source of Fecal Indicator Bacteria (Escherichia coli and Enterococcus)
Escherichia coli Enterococcus
2015/7/21
The purpose of this activity is for students to investigate water quality, and the factors that affect it, at a local beach. This is accomplished by (1) measuring concentrations of Escherichia coli an...