搜索结果: 1-15 共查到“知识库 Sry”相关记录26条 . 查询时间(0.067 秒)
Penile hypoplasia and rudimentary prepuce in a dog (78,XY;SRY-positive):a case report
dog disorder of sex development penile hypoplasia rudimentary prepuce SRY ZFX/ZFY
2016/6/3
This is the first report in the literature concerning penile hypoplasia and rudimentary prepuce in a 78,XY; SRY-positive male that received a successful surgical treatment. A six-month-old male mixed-...
SRY-negative XX sex reversal in an English Cocker Spaniel: a case report
English Cocker Spaniel intersexuality SRY-negative
2015/5/25
A SRY-negative XX male describes a female karyotype with testicular tissue resulting from abnormal
gonadal development. In such cases male gonad formation takes place in the absence of a Y chromosom...
SRY-negative XX sex reversal in an English Cocker Spaniel:a case report
English Cocker Spaniel intersexuality SRY-negative sex determination XX male
2015/4/30
A SRY-negative XX male describes a female karyotype with testicular tissue resulting from abnormal gonadal development. In such cases male gonad formation takes place in the absence of a Y chromosome ...
胃癌组织中 SRY-Box2 基因的表达与其甲基化的关系
SRY-Box2 基因 甲基化 胃癌
2014/4/28
检测干细胞转录因子 SRY-Box2 基因在胃癌MKN74、MKN45细胞株及人胃癌组织标本中的mRNA表达水平与甲基化情况，分析其与胃癌发生及临床病理特征之间的相关性。 方法： 选取河北医科大学第四医院胸外科2007至2012年收治的胃癌患者86例，取癌组织原发灶及其癌旁组织；分别用5-氮杂-2′-脱氧胞苷（5-aza-2′-deoxycytidine，5-Aza-Dc）与曲古抑菌素A（tric...
Detection of the SRY Transcript and Protein in Bovine Ejaculated Spermatozoa
SRY Gene Bovine Spermatozoa In situ Hybridization Bovine
2016/5/9
The sex-determining region on the Y (SRY) gene is important in mammalian sex determination and differentiation. We report a study of the abundance of SRY gene products in bovine ejaculate. RT-PCR expe...
性别决定基因Sry原核表达载体的构建和蛋白检测
性别决定 Sry 原核表达载体 蛋白表达
2009/8/3
动物的性别在畜牧业是一项很重要的经济性状，而哺乳动物的性别决定受睾丸决定基因Sry的启动，获得SRY蛋白并探索它与其他相关因子之间的相互关系对于深入了解该因子的作用机理和寻求高效经济的性别控制方法尤为重要。本研究以小鼠为实验材料，通过PCR、质粒重组、酶切和测序等方法构建小鼠Sry的原核表达载体，使用IPTG诱导蛋白表达、SDS-PAGE电泳和western blotting等方法检测重组表达载体...
利用SRY基因鉴定山羊胎儿成纤维细胞的性别
SRY基因 山羊胎儿成纤维细胞 性别鉴定
2009/7/14
根据GenBank中已发表的山羊SRY基因和β乳球蛋白基因序列设计2对PCR引物，对来自3个1月龄山羊胎儿成纤维细胞的基因组DNA进行PCR扩增，分别以公羊、母羊的基因组DNA为阳性和阴性对照，同时对PCR产物进行序列测定和同源性分析。结果表明，阳性对照和GFF1细胞系有2条扩增带，阴性对照、GFF2和GFF3细胞系只有1条扩增带；序列测定结果表明，PCR扩增产物为SRY基因，利用该方法准确...
Detection of the SRY Gene in a 46,XY Phenotypic Female by the PCR-SSCP Method
PCR-SSCP Sex reversal SRY 46 XY Female
2009/6/30
The short arm of the human Y chromosome determines maleness, initiating the development of the testes. Mutations and deletions in this region affect gonadal differentiation, causing primary sex revers...
Amplification of Porcine SRY Gene for Sex Determination
SRY Gene Sex Determination Sperm Sorting Duplex PCR
2016/4/29
The separation of X and Y chromosome-bearing sperm is of use in many aspects of livestock maintenance. In this study, we sought to determine the difference in DNA content between X- and Y-bearing sper...
Discrimination of Korean Cattle (Hanwoo) Using DNA Markers Derived from SNPs in Bovine Mitochondrial and SRY Genes
Hanwoo Bos taurus Bos indicusx Bos indicus PCR-RFLP SRY ND4 ND5
2016/4/27
In order to distinguish Korean cattle (Hanwoo) beef from the imported beef from Australia in Korean markets, DNA markers based on PCR-RFLP from mitochondrial genes and SRY gene were applied. A total o...
PCR扩增泥鳅和大鳞副泥鳅SRY盒基因
SRY盒 基因 泥鳅 大鳞副泥鳅
2008/3/13
以特异扩增人ＳＲＹ基因保守区的一对引物，研究了泥鳅和大鳞副泥鳅基因组中ＳＲＹ盒基因的扩增。结果表明，该引物可以在泥鳅中扩增出四条带，其长度分别为２００， ５５０、９４０和１０００ｂｐ。在大鳞副泥鳞中扩增出三条带，大小为２００，５５０和９００ｂｐ。经Ｓｏｕｔｈｅｒｎ杂交显示出二者的阳性带为２００和５５０ｂｐ
ｐ。阳性带在雌雄个体间和两个物种间无差异。
摘要应用显微切割技术获得赤麂1号、Y1、Y2染色体，通过DOP-PCR增加模板DNA拷贝数，然后用人的性别决定基因(Sex-determining Region of the Chromosome Y,SRY)中HMG框内设计1对引物，对DOP-PCR产物进行扩增。在雄性赤麂Y2染色体DOP-PCR产物中扩增出与人SRY基因同源的Sry基因片段，克隆、测序，首次在分子水平上证明赤麂Y2染色体是真正...
摘要通过PCR扩增、测序,得到了白臀叶猴和红面猴的SRY基因全序列。结合现有的灵长类其他物种序列进行分析,验证了HMG盒的保守性。通过构建系统发育树,比较旧大陆猴和人猿超科两个类群内和类群间HMG盒侧翼序列K a/Ks的比率。有趣的是,人猿超科两物种比较呈现较高的Ka/Ks比值,但在旧大陆猴中及旧大陆猴与狨猴间的Ka/Ks比值显著低于人猿超科的,呈现很不同的格局。同时,对于HMG盒序列,Ka/Ks...
摘要Y染色体上的性别决定区域——SRY基因作为睾丸决定因子,可以调控男性性别发育过程。SRY基因是一种转录因子，属于带有高迁移率族蛋白家族，该家族成员的包含能与DNA结合的HMG盒基序。已知SRY基因的缺失和点突变是造成XY女性性反转的病因之一。通过筛查10位中国46，XY女性性反转病人SRY基因的开放阅读框区域，探寻新的突变类型。用标准方法从外周血中抽提gDNA，通过聚合酶链式反应扩增SRY基因...